Milman N, Bentzon M W, Graudal N, Juul-Jørgensen B
Department of Medicine Y and Clinical Chemistry, Gentofte Hospital, Copenhagen.
Dan Med Bull. 1993 Sep;40(4):508-10.
Two commercial ferritin kits, Phadebas Ferritin PRIST (kit B) and Ferritin RIA Amersham (kit A) were compared in order to "translate" ferritin values from one kit to the other. Ferritin levels in 222 sera were determined with both kits in the concentration range 5-838 micrograms/l. Regression analysis disclosed a parabolic regression between the logarithmically transformed results obtained with the two kits. Measured kit B values of 10, 12, 15, 30 and 300 micrograms/l corresponded to calculated kit A values of 15, 17, 20, 34 and 321 micrograms/l, respectively. In kit A, storage of sera for two years at -25 degrees C in combination with freeze-thawing three times produced a minor fall (p < 0.0001) in ferritin levels with a median percent decline of 16%.
为了将一种试剂盒的铁蛋白值“转换”为另一种试剂盒的铁蛋白值，对两种商用铁蛋白检测试剂盒——Phadebas铁蛋白PRIST试剂盒（试剂盒B）和Amersham铁蛋白放射免疫分析试剂盒（试剂盒A）进行了比较。用这两种试剂盒测定了222份血清中铁蛋白水平，浓度范围为5 - 838微克/升。回归分析显示，两种试剂盒对数转换后的结果呈抛物线回归。试剂盒B测得的10、12、15、30和300微克/升的值分别对应于试剂盒A计算得出的15、17、20、34和321微克/升的值。在试剂盒A中，血清在-25℃下储存两年并冻融三次后，铁蛋白水平出现轻微下降（p < 0.0001），中位数下降百分比为16%。
