Oliveira R S, Vosátka M, Dodd J C, Castro P M L
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal.
Institute of Botany, Academy of Sciences of the Czech Republic, 252 43, Pruhonice, Czech Republic.
Mycorrhiza. 2005 Dec;16(1):23-31. doi: 10.1007/s00572-005-0010-0. Epub 2005 Nov 11.
A field survey of the arbuscular mycorrhizal status of herbaceous plant species was conducted in a highly alkaline anthropogenic sediment resulting from the disposal of waste from an acetylene and polyvinyl chloride factory. Most plant species found at the site were mycorrhizal and the dominant mycotrophic plant species was Conyza bilbaoana. Fungal species richness was assessed by identification of spores extracted from the sediment and from continuously propagated trap pot cultures. All of the six species of arbuscular mycorrhizal fungi (AMF) found were from the genus Glomus. Glomus intraradices and G. mosseae were found in field-collected sediment samples and also occurred most frequently in trap cultures. To test the symbiotic effectiveness of these two fungi, seedlings of C. bilbaoana were inoculated with either native G. intraradices BEG163 or G. mosseae BEG198 and non-native G. intraradices BEG75 or G. mosseae BEG25 isolates in sterile and non-sterile sediment collected from the study site. All four isolates were able to colonise C. bilbaoana. However, AMF native to the target sediments were generally more effective than the non-native fungi in promoting plant establishment and growth under highly alkaline conditions. The non-native G. intraradices was, however, more effective than the non-native G. mosseae. The results of this study suggest the use of adapted AMF as inoculants for phytorestoration of alkaline anthropogenic-stressed sediments.
对乙炔和聚氯乙烯工厂废弃物处置产生的高碱性人为沉积物中草本植物物种的丛枝菌根状况进行了实地调查。在该场地发现的大多数植物物种都有菌根，优势菌根营养植物物种是毕氏飞蓬。通过鉴定从沉积物和连续繁殖的诱捕盆栽培养物中提取的孢子来评估真菌物种丰富度。发现的所有六种丛枝菌根真菌（AMF）均属于球囊霉属。在野外采集的沉积物样本中发现了根内球囊霉和摩西球囊霉，并且在诱捕培养物中也最常出现。为了测试这两种真菌的共生有效性，在从研究地点采集的无菌和非无菌沉积物中，用本地根内球囊霉BEG163或摩西球囊霉BEG198以及非本地根内球囊霉BEG75或摩西球囊霉BEG25分离株接种毕氏飞蓬幼苗。所有四种分离株都能够定殖于毕氏飞蓬。然而，在高碱性条件下，目标沉积物中的本地AMF在促进植物定植和生长方面通常比非本地真菌更有效。不过，非本地根内球囊霉比非本地摩西球囊霉更有效。本研究结果表明，可使用适应性的AMF作为接种剂用于碱性人为胁迫沉积物的植物修复。
