Tsai Dong-Mung, Lin Kuan-Wen, Zen Jyh-Myng, Chen Hung-Yu, Hong Ray-Hua
Department of Chemistry, National Chung Hsing University, Taichung, Taiwan.
Electrophoresis. 2005 Aug;26(15):3007-12. doi: 10.1002/elps.200500107.
We report here a novel and simple process for the fabrication of a poly(methyl methacrylate) (PMMA)-based microchip electrophoresis device, integrated with a screen-printed three-electrode electrochemical detector that does not require a replicate mold. In this approach, a photoresist layer constitutes both an adhesion layer and side walls of 50 mum wide and 50 mum tall microfluidic channels on a screen-printed three-electrode PMMA substrate. Openings were drilled for buffer reservoirs on an additional piece of PMMA, then the final device was bonded in a PMMA/photoresist/PMMA sandwich configuration. This process is inexpensive, less time-consuming, and simpler compared with traditional fabrication methods. The combination of this PMMA-based microchip fabrication together with screen-printed electrode technology holds great promise for the mass production of a single-use micrototal analytical system. Successful determination of uric acid and L-ascorbic acid with the presented system validates its utility. In combination with a suitable electrochemical detector, this device holds much promise for the determination of other analytes in various biological samples for medical and clinical diagnosis.
我们在此报告一种新颖且简单的方法，用于制造基于聚甲基丙烯酸甲酯（PMMA）的微芯片电泳装置，该装置集成了无需复制模具的丝网印刷三电极电化学检测器。在这种方法中，光刻胶层在丝网印刷的三电极PMMA基板上既构成粘附层，又构成宽50微米、高50微米的微流体通道的侧壁。在另一块PMMA上钻出缓冲液储槽的开口，然后将最终装置以PMMA/光刻胶/PMMA三明治结构进行键合。与传统制造方法相比，该方法成本低廉、耗时更少且更简单。这种基于PMMA的微芯片制造与丝网印刷电极技术相结合，对于一次性微全分析系统的大规模生产具有很大的前景。所展示的系统成功测定尿酸和L-抗坏血酸，验证了其实用性。与合适的电化学检测器相结合，该装置在医学和临床诊断中用于测定各种生物样品中的其他分析物具有很大潜力。
