Tsai Yuan-Chien, Jen Hsiu-Ping, Lin Kuan-Wen, Hsieh You-Zung
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan.
J Chromatogr A. 2006 Apr 14;1111(2):267-71. doi: 10.1016/j.chroma.2005.12.003. Epub 2005 Dec 27.
An inexpensive, disposable microfluidic device was fabricated from a dry film photoresist using a combination of photolithographic and hot roll lamination techniques. A microfluidic flow pattern was prefabricated in a dry film photoresist tape using traditional photolithographic methods. This tape became bonded to a poly(methyl methacrylate) (PMMA) sheet with prepouched holes when passed through a hot roll laminator. A copper working electrode and platinum decoupler was readily incorporated within this microchip. The integrated microchip device was then fixed in a laboratory-built Plexiglas holder prior to its use in microchip capillary electrophoresis. The performance of this device with amperometric detection for the separation of dopamine and catechol was examined. The separation was complete within 50 s at an applied potential of 200 V/cm. The relative standard deviations (RSD) of analyte migration times were less than 0.71%, and the theoretical plate numbers for dopamine and catechol were 3.2 x 10(4) and 4.1 x 10(4), respectively, based on a 65 mm separation channel.
使用光刻和热辊层压技术相结合的方法，由干膜光刻胶制造出一种廉价的一次性微流控装置。采用传统光刻方法在干膜光刻胶带中预制微流控流动模式。当该胶带通过热辊层压机时，会与带有预冲孔的聚甲基丙烯酸甲酯（PMMA）片材粘合在一起。铜工作电极和铂去耦器很容易集成到这个微芯片中。然后，在将集成微芯片装置用于微芯片毛细管电泳之前，将其固定在实验室自制的有机玻璃支架中。考察了该装置在安培检测下分离多巴胺和儿茶酚的性能。在200 V/cm的施加电位下，50秒内分离完成。基于65毫米的分离通道，分析物迁移时间的相对标准偏差（RSD）小于0.71%，多巴胺和儿茶酚的理论塔板数分别为3.2×10⁴和4.1×10⁴。
