Didier R, Meiniel R, Meiniel A
Laboratoire de Biochimie médicale, CJF INSERM 88.06, Université d'Auvergne, Clermont-Ferrand, France.
Dev Neurosci. 1992;14(1):44-52. doi: 10.1159/000111646.
Monoclonal antibodies directed against components of the subcommissural organ (SCO) of the chick embryo were produced by immunizing mice with SCO homogenate. In three series of production, 788 hybridomas were screened by immunofluorescence microscopy. Four hybridoma cell lines producing antibodies that specifically recognize both SCO cells and Reissner's fiber (RF) were selected and cloned. Using these immunological probes, the ontogenetic development of the SCO and RF was investigated in the chick embryo. Immunoreactive material could be detected in the SCO anlage from stage 17 on and RF was first observed in the central canal of the thoracal part of the spinal cord in 10-day-old embryos. Monoclonal antibodies can be useful as markers for analyzing molecular mechanisms involved in the specific function of these ependymal cells.
通过用鸡胚室管膜下器官（SCO）匀浆免疫小鼠，制备了针对该器官成分的单克隆抗体。在三个系列的制备过程中，通过免疫荧光显微镜筛选了788个杂交瘤。选择并克隆了四个产生特异性识别SCO细胞和赖氏纤维（RF）抗体的杂交瘤细胞系。利用这些免疫探针，研究了鸡胚中SCO和RF的个体发生发育。从第17阶段开始，在SCO原基中可检测到免疫反应性物质，在10日龄胚胎的脊髓胸段中央管中首次观察到RF。单克隆抗体可作为分析这些室管膜细胞特定功能所涉及分子机制的标志物。
