[多囊卵巢综合征所致不孕症女性未刺激周期卵母细胞的体外成熟与受精]

[In vitro maturation and fertilization of oocytes from unstimulated cycles in women with infertility due to polycystic ovary syndrome].

作者信息

Li Yuan, Jiang Jing-jing, Ma Shui-ying, Li Mei, Hu Jing-mei, Zhao Li-xin, Chen Zi-jiang

机构信息

Reproductive Medicine Center of Shandong Provincial Hospital, Shandong University, Jinan 250021, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2005 Jun;40(6):388-91.

PMID:16008889

Abstract

OBJECTIVE

To investigate the effect of in vitro maturation (IVM) and fertilization of oocytes from unstimulated cycles in women with infertility due to polycystic ovary syndrome (PCOS).

METHODS

Seventy women with PCOS, who came to our clinic during March to were involved in this trial. June 2003. Every cycle was given human chorionic gonadotropin 10,000 IU 36 h before oocyte retrieval. Immature oocytes were matured in vitro and fertilized, and the resulting embryos were replaced back to the uterus.

RESULTS

A total of 94 IVM cycles were performed and 1283 oocytes were obtained. The overall maturation, fertilization and cleavage rates were 65.3%, 66.0% and 48.0% respectively. Maturation, fertilization, cleavage and high quality embryo rates had no difference between metaphase I stage oocytes (69.7%, 71.7%, 52.2% and 26.1% respectively) and germinal vesical (GV) stage oocytes (67.7%, 66.4%, 47.6% and 24.1% respectively), but the rates in those oocytes which could not be classified (44.8%, 53.8%, 46.2% and 16.9% respectively) were much lower than the former two groups. After embryo transfer, 18 pregnancies were reported (24%).

CONCLUSION

IVM/IVF-ET in unstimulated cycles is a feasible treatment for women with PCOS.

摘要

目的

探讨多囊卵巢综合征（PCOS）所致不孕症患者未刺激周期卵母细胞的体外成熟（IVM）及受精效果。

方法

2003年3月至6月期间，70例PCOS患者到我院参与本试验。每个周期在取卵前36小时给予10000IU人绒毛膜促性腺激素。未成熟卵母细胞进行体外成熟及受精，然后将所得胚胎移植回子宫。

结果

共进行了94个IVM周期，获取了1283枚卵母细胞。总体成熟率、受精率和卵裂率分别为65.3%、66.0%和48.0%。中期I期卵母细胞（分别为69.7%、71.7%、52.2%和26.1%）和生发泡（GV）期卵母细胞（分别为67.7%、66.4%、47.6%和24.1%）的成熟率、受精率、卵裂率和优质胚胎率无差异，但无法分类的卵母细胞（分别为44.8%、53.8%、46.2%和16.9%）的这些比率远低于前两组。胚胎移植后，报告了18例妊娠（24%）。