Caldefie-Chézet F, Damez M, de Latour M, Konska G, Mishellani F, Fusillier C, Guerry M, Penault-Llorca F, Guillot J, Vasson M-P
L.B.C.M., EA 2416, Centre de Recherche en Nutrition Humaine Auvergne, Faculté de Pharmacie, France.
Biochem Biophys Res Commun. 2005 Sep 2;334(3):737-41. doi: 10.1016/j.bbrc.2005.06.077.
Mammary adipose tissue is an important source of paracrine mitogens and anti-mitogens, including insulin-like growth factor, transforming growth factors, and cytokines (especially, TNFalpha and IL-1beta). Nevertheless, it is also an important source of the adipocytokine, leptin. Recently, leptin was reported to stimulate the proliferation of various cell types (pancreatic beta cells, prostate, colorectal, lung, etc.) as a new growth factor. It was also shown to stimulate the proliferation of breast cancer cell lines. In this study, we conducted an immunohistochemical analysis of leptin expression in normal tissue and benign and malignant ductal breast cell, representing the different states of the invasion process. We determined for the first time that leptin is expressed both by ductal breast tumors and by benign lesions as atypical hyperplasia. This suggests that leptin may be taken up or synthesized by all modified ductal breast cells, and may prove a proliferative factor. Moreover, leptin is unexpressed by normal tissue in the healthy breast but is exhibited by the normal tissue in near vicinity of the malignant ductal breast lesions. We also postulated that leptin may be a prognostic or diagnostic factor for ductal breast cancer. These putative hypotheses require further study.
乳腺脂肪组织是旁分泌促有丝分裂原和抗有丝分裂原的重要来源，包括胰岛素样生长因子、转化生长因子和细胞因子（特别是肿瘤坏死因子α和白细胞介素-1β）。然而，它也是脂肪细胞因子瘦素的重要来源。最近，有报道称瘦素作为一种新的生长因子可刺激多种细胞类型（胰腺β细胞、前列腺、结肠、肺等）的增殖。研究还表明它能刺激乳腺癌细胞系的增殖。在本研究中，我们对瘦素在正常组织以及代表侵袭过程不同状态的乳腺导管良性和恶性细胞中的表达进行了免疫组化分析。我们首次确定瘦素在乳腺导管肿瘤和作为非典型增生的良性病变中均有表达。这表明瘦素可能被所有乳腺导管修饰细胞摄取或合成，并可能是一种增殖因子。此外，瘦素在健康乳腺的正常组织中不表达，但在乳腺导管恶性病变附近的正常组织中表达。我们还推测瘦素可能是乳腺导管癌的一个预后或诊断因子。这些假定的假说需要进一步研究。
