Le Bourvellec C, Renard C M G C
Unité de Recherches Cidricoles, Biotransformation des Fruits et Légumes, INRA, 35653 Le Rheu Cédex, France.
Biochim Biophys Acta. 2005 Aug 30;1725(1):1-9. doi: 10.1016/j.bbagen.2005.06.003.
The adsorption of condensed tannins (procyanidins) on solid cell wall material was quantified using the Langmuir isotherms formulation. Six tannins fractions differing by their size (number average degree of polymerisation between 2.5 and 65) and composition (presence of galloyl groups from to 0 to 22%, proportions of (+)-catechin to (-)-epicatechin from traces to one CAT for three EPI) were used. Two cell walls differing only by their physical characteristics were obtained by mild or harsh drying, with surface areas of 2.15 and 0.52 m(2)/g, respectively. The total amounts of procyanidins retained on the cell wall materials increased with their concentrations while the proportions of retained decreased, and a plateau was reached at high concentrations. The apparent affinity of procyanidins for CWM isolated from apples increased with their molecular weight. Decrease of the CWM porosity by harsh drying slightly decreased the apparent affinity and increased the apparent saturation levels when constants were expressed relative to cell wall weight, but strongly increased both apparent affinity and apparent saturation levels per surface units.
使用朗缪尔等温线公式对缩合单宁（原花青素）在固体细胞壁材料上的吸附进行了定量分析。使用了六种单宁组分，它们在大小（聚合数均度在2.5至65之间）和组成上有所不同（没食子酰基的含量从0至22%，（+）-儿茶素与（-）-表儿茶素的比例从痕量到三个表儿茶素对应一个儿茶素）。通过温和或剧烈干燥获得了仅在物理特性上有所不同的两种细胞壁，其表面积分别为2.15和0.52平方米/克。保留在细胞壁材料上的原花青素总量随其浓度增加而增加，而保留比例下降，在高浓度时达到平稳状态。原花青素对从苹果中分离出的细胞壁材料的表观亲和力随其分子量增加而增加。当以细胞壁重量为基准表示常数时，剧烈干燥导致细胞壁孔隙率降低，这略微降低了表观亲和力并提高了表观饱和水平，但以每表面单位表示时，表观亲和力和表观饱和水平均大幅提高。
