Renard C M, Baron A, Guyot S, Drilleau J F
Unité de Recherches Cidricoles-Biotransformation des Fruits et Légumes, I.N.R.A., B.P. 35327, 35653 Le Rheu Cédex, France.
Int J Biol Macromol. 2001 Aug 20;29(2):115-25. doi: 10.1016/s0141-8130(01)00155-6.
Cell walls were prepared from apple parenchyma by a phenol:buffer procedure. Polyphenols were extracted from freeze-dried apple parenchyma by methanol and water:acetone, and purified by preparative HPLC. Interactions were quantified by bringing into contact suspended cell walls and polyphenol solutions. Hydroxycinnamic acids and (-)-epicatechin did not bind to cell walls. Binding of procyanidins was fast and reached up to 0.6 g per g cell walls. The amounts of procyanidins bound increased with the initial concentration and with DPn. Procyanidins could be partially desorbed by buffer, more being desorbed in the presence of dissolved cell wall polysaccharides. They were totally desorbed using 8 M urea or acetone:water. Higher polymers were bound selectively from procyanidin mixtures, and very high average DPn were obtained in extensively washed complexes. Binding of procyanidins inhibited enzymic degradation of the cell walls.
缓冲液法从苹果薄壁组织制备细胞壁。用甲醇以及水：丙酮从冻干的苹果薄壁组织中提取多酚，并通过制备型高效液相色谱法进行纯化。通过使悬浮的细胞壁与多酚溶液接触来对相互作用进行定量。羟基肉桂酸和（-）-表儿茶素不与细胞壁结合。原花青素的结合很快，每克细胞壁可达0.6克。结合的原花青素的量随初始浓度和聚合度（DPn）的增加而增加。原花青素可以被缓冲液部分解吸，在溶解的细胞壁多糖存在下更多的原花青素会被解吸。使用8M尿素或丙酮：水可将它们完全解吸。从原花青素混合物中选择性地结合更高的聚合物，并且在充分洗涤的复合物中获得非常高的平均聚合度。原花青素的结合抑制了细胞壁的酶促降解。
