Gustafsson Asa B, Gottlieb Roberta A, Granville David J
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.
Methods Mol Med. 2005;112:81-90.
TAT protein transduction is a novel method of delivering biologically active proteins into cells and tissues through the fusion of a protein transduction domain to the protein of interest. The present chapter outlines the methodology pertaining to the preparation of TAT-fusion proteins and how to efficiently transduce these proteins into cultured cells or isolated rat hearts perfused in Langendorff mode.
TAT蛋白转导是一种通过将蛋白转导结构域与目标蛋白融合，从而将生物活性蛋白递送至细胞和组织的新方法。本章概述了与TAT融合蛋白制备相关的方法，以及如何将这些蛋白有效地转导至培养细胞或采用Langendorff模式灌注的离体大鼠心脏中。
