Zeineddine Dana, Papadimou Evangelia, Mery Annabelle, Ménard Claudine, Pucéat Michel
Centre de Recherches de Biochimie Macromoléculaire, Montpellier, France.
Methods Mol Med. 2005;112:175-82.
Embryonic stem (ES) cells represent a source for cell-based regenerative therapies of heart failure. The pluripotency and the plasticity of ES cells allow them to be committed to a cardiac lineage following treatment with growth factors of the transforming growth factor (TGF)-beta superfamily. We describe a protocol designed to turn on expression of cardiac-specific genes in undifferentiated murine ES cells stimulated with BMP2 and/or TGF-beta. Cell commitment results in a significant improvement in spontaneous cardiac differentiation of ES cells both in vitro and in vivo.
胚胎干细胞（ES细胞）是心力衰竭基于细胞的再生疗法的一个来源。ES细胞的多能性和可塑性使它们在用转化生长因子（TGF）-β超家族的生长因子处理后能够定向分化为心脏谱系。我们描述了一种方案，旨在开启用骨形态发生蛋白2（BMP2）和/或TGF-β刺激的未分化小鼠ES细胞中心脏特异性基因的表达。细胞定向分化导致ES细胞在体外和体内的自发性心脏分化有显著改善。
