• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于两种不同 miRNA 芯片平台的鼠胚胎干细胞心肌细胞特异性分化过程中的 microRNA 图谱分析。

MicroRNA profiling during cardiomyocyte-specific differentiation of murine embryonic stem cells based on two different miRNA array platforms.

机构信息

Interdisciplinary Centre for Clinical Research Aachen, RWTH Aachen University, Aachen, Germany.

出版信息

PLoS One. 2011;6(10):e25809. doi: 10.1371/journal.pone.0025809. Epub 2011 Oct 3.

DOI:10.1371/journal.pone.0025809
PMID:21991358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3185008/
Abstract

MicroRNA (miRNA) plays a critical role in a wide variety of biological processes. Profiling miRNA expression during differentiation of embryonic stem cells will help to understand the regulation pathway of differentiation, which in turn may elucidate disease mechanisms. The identified miRNAs could then serve as a new group of possible therapeutic targets. In the present paper, miRNA expression profiles were determined during cardiomyocyte-specific differentiation and maturation of murine embryonic stem (ES) cells. For this purpose a homogeneous cardiomyocyte population was generated from a transgenic murine ES cell line. Two high throughput array platforms (Affymetrix and Febit) were used for miRNA profiling in order to compare the effect of the platforms on miRNA profiling as well as to increase the validity of target miRNA identification. Four time points (i.e. day 0, day 12, day 19 and day 26) were chosen for the miRNA profiling study, which corresponded to different stages during cardiomyocyte-specific differentiation and maturation. Fifty platform and pre-processing method-independent miRNAs were identified as being regulated during the differentiation and maturation processes. The identification of these miRNAs is an important step for characterizing and understanding the events involved in cardiomyocyte-specific differentiation of ES cells and may also highlight candidate target molecules for therapeutic purposes.

摘要

微小 RNA（miRNA）在广泛的生物过程中起着至关重要的作用。对胚胎干细胞分化过程中的 miRNA 表达谱进行分析，有助于理解分化的调控途径，进而可能阐明疾病的发病机制。鉴定出的 miRNAs 随后可能成为一组新的可能的治疗靶点。本研究旨在确定小鼠胚胎干细胞（ES 细胞）向心肌细胞特异性分化和成熟过程中的 miRNA 表达谱。为此，我们从一个转基因的小鼠 ES 细胞系中生成了一个同质的心肌细胞群体。使用两种高通量微阵列平台（Affymetrix 和 Febit）进行 miRNA 分析，以比较平台对 miRNA 分析的影响，从而提高靶 miRNA 鉴定的有效性。选择了四个时间点（即第 0 天、第 12 天、第 19 天和第 26 天）进行 miRNA 分析，这对应于心肌细胞特异性分化和成熟过程中的不同阶段。鉴定出 50 个与平台和预处理方法无关的 miRNA 在分化和成熟过程中受到调控。鉴定这些 miRNA 是对 ES 细胞向心肌细胞特异性分化过程中涉及的事件进行特征描述和理解的重要步骤，也可能突出治疗目的的候选靶分子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/d2935a6b590c/pone.0025809.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/73c212454f7b/pone.0025809.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/e6400120e12d/pone.0025809.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/319ea4316637/pone.0025809.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/490c1f558916/pone.0025809.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/8b22344063a0/pone.0025809.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/367dd3b8553a/pone.0025809.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/74f1bfa9626b/pone.0025809.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/ab71506d2193/pone.0025809.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/853b9c32f88c/pone.0025809.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/d2935a6b590c/pone.0025809.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/73c212454f7b/pone.0025809.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/e6400120e12d/pone.0025809.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/319ea4316637/pone.0025809.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/490c1f558916/pone.0025809.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/8b22344063a0/pone.0025809.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/367dd3b8553a/pone.0025809.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/74f1bfa9626b/pone.0025809.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/ab71506d2193/pone.0025809.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/853b9c32f88c/pone.0025809.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea3/3185008/d2935a6b590c/pone.0025809.g010.jpg

相似文献

1
MicroRNA profiling during cardiomyocyte-specific differentiation of murine embryonic stem cells based on two different miRNA array platforms.基于两种不同 miRNA 芯片平台的鼠胚胎干细胞心肌细胞特异性分化过程中的 microRNA 图谱分析。
PLoS One. 2011;6(10):e25809. doi: 10.1371/journal.pone.0025809. Epub 2011 Oct 3.
2
Expression of microRNAs and their target mRNAs in human stem cell-derived cardiomyocyte clusters and in heart tissue.人干细胞源性心肌细胞集落和心脏组织中 microRNAs 及其靶 mRNAs 的表达。
Physiol Genomics. 2011 May 1;43(10):581-94. doi: 10.1152/physiolgenomics.00074.2010. Epub 2010 Sep 14.
3
MicroRNA expression profiling of oligodendrocyte differentiation from human embryonic stem cells.人胚胎干细胞向少突胶质细胞分化过程中的 microRNA 表达谱分析。
PLoS One. 2010 May 5;5(5):e10480. doi: 10.1371/journal.pone.0010480.
4
Transcriptome analysis in cardiomyocyte-specific differentiation of murine embryonic stem cells reveals transcriptional regulation network.小鼠胚胎干细胞心肌细胞特异性分化中的转录组分析揭示了转录调控网络。
Gene Expr Patterns. 2014 Sep;16(1):8-22. doi: 10.1016/j.gep.2014.07.002. Epub 2014 Jul 21.
5
Differential expression of microRNAs in cardiac myocytes compared to undifferentiated P19 cells.心肌细胞与未分化 P19 细胞相比的 microRNAs 差异表达。
Int J Mol Med. 2011 Jul;28(1):59-64. doi: 10.3892/ijmm.2011.664. Epub 2011 Mar 31.
6
Improving murine embryonic stem cell differentiation into cardiomyocytes with neuregulin-1: differential expression of microRNA.神经调节蛋白 1 对鼠胚胎干细胞向心肌细胞分化的影响：microRNA 的差异表达。
Am J Physiol Cell Physiol. 2011 Jul;301(1):C21-30. doi: 10.1152/ajpcell.00141.2010. Epub 2011 Mar 30.
7
Determination of the human cardiomyocyte mRNA and miRNA differentiation network by fine-scale profiling.通过精细分析确定人类心肌细胞 mRNA 和 miRNA 分化网络。
Stem Cells Dev. 2012 Jul 20;21(11):1956-65. doi: 10.1089/scd.2011.0357. Epub 2012 Jan 4.
8
MicroRNA regulation and role in stem cell maintenance, cardiac differentiation and hypertrophy.微小 RNA 的调控作用及其在干细胞维持、心脏分化和肥大中的作用。
Curr Mol Med. 2013 Jun;13(5):757-64. doi: 10.2174/1566524011313050007.
9
MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells.人类胚胎干细胞分化过程中的微小RNA与基因表达模式
J Transl Med. 2009 Mar 23;7:20. doi: 10.1186/1479-5876-7-20.
10
Wnt2 accelerates cardiac myocyte differentiation from ES-cell derived mesodermal cells via non-canonical pathway.Wnt2 通过非经典途径加速 ES 细胞来源的中胚层细胞向心肌细胞的分化。
J Mol Cell Cardiol. 2012 Mar;52(3):650-9. doi: 10.1016/j.yjmcc.2011.11.010. Epub 2011 Nov 29.

引用本文的文献

1
Let-7 family of microRNA is required for maturation and adult-like metabolism in stem cell-derived cardiomyocytes.微小RNA的Let-7家族对于干细胞衍生的心肌细胞的成熟和类成体代谢是必需的。
Proc Natl Acad Sci U S A. 2015 May 26;112(21):E2785-94. doi: 10.1073/pnas.1424042112. Epub 2015 May 11.
2
In vitro application of ribonucleases: comparison of the effects on mRNA and miRNA stability.核糖核酸酶的体外应用：对信使核糖核酸和微小核糖核酸稳定性影响的比较
BMC Res Notes. 2015 Apr 22;8:164. doi: 10.1186/s13104-015-1114-z.
3
Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression.

本文引用的文献

1
MiRror: a combinatorial analysis web tool for ensembles of microRNAs and their targets.MiRror：一个用于 miRNA 及其靶基因组合分析的网络工具。
Bioinformatics. 2010 Aug 1;26(15):1920-1. doi: 10.1093/bioinformatics/btq298. Epub 2010 Jun 6.
2
Network analysis of primary hepatocyte dedifferentiation using a shotgun proteomics approach.基于蛋白质组学的高通量技术分析原代肝细胞去分化。
J Proteome Res. 2010 May 7;9(5):2658-68. doi: 10.1021/pr1001687.
3
MicroRNAs in cardiac development.心脏发育中的微小RNA
组织力学调节 microRNA 依赖性 PTEN 表达以调节恶性进展。
Nat Med. 2014 Apr;20(4):360-7. doi: 10.1038/nm.3497. Epub 2014 Mar 16.
4
MicroRNA regulation and role in stem cell maintenance, cardiac differentiation and hypertrophy.微小 RNA 的调控作用及其在干细胞维持、心脏分化和肥大中的作用。
Curr Mol Med. 2013 Jun;13(5):757-64. doi: 10.2174/1566524011313050007.
5
MicroRNAs: a new piece in the paediatric cardiovascular disease puzzle.微小RNA：小儿心血管疾病谜题中的新拼图。
Cardiol Young. 2013 Oct;23(5):642-55. doi: 10.1017/S1047951113000048. Epub 2013 Feb 26.
6
Temporal- and strain-specific host microRNA molecular signatures associated with swine-origin H1N1 and avian-origin H7N7 influenza A virus infection.与猪源 H1N1 和禽流感 H7N7 甲型流感病毒感染相关的具有时间和株特异性的宿主 microRNA 分子特征。
J Virol. 2012 Jun;86(11):6109-22. doi: 10.1128/JVI.06892-11. Epub 2012 Mar 21.
Pediatr Cardiol. 2010 Apr;31(3):349-56. doi: 10.1007/s00246-010-9639-3. Epub 2010 Feb 7.
4
The mechanism of eukaryotic translation initiation and principles of its regulation.真核生物翻译起始的机制与调控原则。
Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27. doi: 10.1038/nrm2838.
5
Embryonic and adult stem cell systems in mammals: ontology and regulation.哺乳动物的胚胎和成人干细胞系统：本体论和调控。
Dev Growth Differ. 2010 Jan;52(1):115-29. doi: 10.1111/j.1440-169X.2009.01160.x.
6
Gene expression in stem cells.干细胞中的基因表达。
Crit Rev Eukaryot Gene Expr. 2009;19(4):289-300. doi: 10.1615/critreveukargeneexpr.v19.i4.30.
7
Controllable expansion of primary cardiomyocytes by reversible immortalization.可控扩张原代心肌细胞的可逆永生化。
Hum Gene Ther. 2009 Dec;20(12):1687-96. doi: 10.1089/hum.2009.057.
8
Analysis of epigenetic alterations to chromatin during development.发育过程中染色质表观遗传改变的分析。
Genesis. 2009 Aug;47(8):559-72. doi: 10.1002/dvg.20534.
9
MicroRNA and stem cell regulation.微小RNA与干细胞调控
Curr Opin Mol Ther. 2009 Jun;11(3):292-8.
10
A review of gene expression profiling of human embryonic stem cell lines and their differentiated progeny.人类胚胎干细胞系及其分化后代的基因表达谱综述。
Curr Stem Cell Res Ther. 2009 May;4(2):98-106. doi: 10.2174/157488809788167409.