Apoil Pol André, Puissant Bénédicte, Roubinet Francis, Abbal Michel, Massip Patrice, Blancher Antoine
Laboratoire d'Immunogénétique Moléculaire, Faculté de Médecine de Rangueil, Toulouse, France.
J Acquir Immune Defic Syndr. 2005 Aug 1;39(4):381-5. doi: 10.1097/01.qai.0000169662.30783.2d.
The impact of HIV infection on regulatory CD4(+)CD25(high) (Treg) lymphocyte subpopulations was evaluated by FOXP3 quantitative reverse transcriptase polymerase chain reaction and by flow cytometry. FOXP3 mRNA was quantified in peripheral blood mononuclear cells or purified CD4(+) lymphocytes from HIV(+) lymphopenic patients. Patients were distributed among clinical stages A, B, and C and received highly active antiretroviral therapy. The frequency of CD4(+)CD25(high) lymphocytes, measured by flow cytometry, was decreased in HIV patients (n = 38) compared with the group of uninfected subjects (n = 39). FOXP3 mRNA levels were found decreased in HIV patients (n = 25) compared with controls (n = 17) when expression of CD3gamma or beta-actin but not that of TATA box binding protein 1 was used for data normalization. Our results are compatible with a decrease of the Treg lymphocytes during HIV infection. The consequences of a Treg decrease are discussed in the context of immunologic anomalies observed during HIV infection.
通过FOXP3定量逆转录聚合酶链反应和流式细胞术评估了HIV感染对调节性CD4(+)CD25(高)（Treg）淋巴细胞亚群的影响。对HIV(+)淋巴细胞减少患者外周血单个核细胞或纯化的CD4(+)淋巴细胞中的FOXP3 mRNA进行定量。患者被分为临床A、B和C期，并接受高效抗逆转录病毒治疗。与未感染组（n = 39）相比，通过流式细胞术检测发现HIV患者（n = 38）中CD4(+)CD25(高)淋巴细胞的频率降低。当使用CD3γ或β-肌动蛋白而非TATA盒结合蛋白1的表达进行数据标准化时，发现HIV患者（n = 25）与对照组（n = 17）相比，FOXP3 mRNA水平降低。我们的结果与HIV感染期间Treg淋巴细胞减少相符。在HIV感染期间观察到的免疫异常背景下讨论了Treg减少的后果。
