Fangmann J, Dalchau R, Sawyer G J, Priestley C A, Fabre J W
Division of Cell and Molecular Biology, University of London, GB.
Eur J Immunol. 1992 Jun;22(6):1525-30. doi: 10.1002/eji.1830220627.
LEW (RTI1) recipients of DA (RTIav1) skin and kidney allografts were tested for the capacity of their T lymphocytes to proliferate to three 22-24-amino acid peptides from the hypervariable regions of the RTI-Aav1 classical MHC class I molecule. Ten days after rejecting second-set DA kidney allografts, spleen cells (but interestingly not lymph node cells) from LEW recipients showed strong, LEW antigen-presenting cell-dependent, CD4+ T cell proliferation to peptide 1 (from the alpha helical region of the alpha 1 domain). CD8+ T cells showed no response to peptide 1. There was no response by the spleen cells to peptide 2 (from the beta sheet of the alpha 2 domain) or peptide 3 (from the alpha helical region of the alpha 2 domain). Immunization of LEW rats with pure RTI-Aav1 class I H chain in Freund's adjuvant gave responses identical to that seen after grafting, i.e. good CD4+ T cell proliferation to peptide 1, but none to peptides 2 and 3. However, immunization of LEW rats with peptides 1, 2 and 3 in Freund's adjuvant resulted in good CD4+ T cell proliferation responses to each of the peptides. These data demonstrate that indirect allorecognition can be stimulated by allograft rejection, and emphasize that the physiological processing of donor antigens will influence which peptides will be important in indirect allorecognition in transplantation.
对接受DA（RTIav1）皮肤和肾脏同种异体移植的LEW（RTI1）受体，检测其T淋巴细胞对来自RTI-Aav1经典MHC I类分子高变区的三种22 - 24个氨基酸肽的增殖能力。在排斥第二组DA肾脏同种异体移植10天后，LEW受体的脾细胞（但有趣的是淋巴结细胞没有）表现出强烈的、依赖LEW抗原呈递细胞的CD4⁺ T细胞对肽1（来自α1结构域的α螺旋区）的增殖。CD8⁺ T细胞对肽1无反应。脾细胞对肽2（来自α2结构域的β折叠）或肽3（来自α2结构域的α螺旋区）无反应。用弗氏佐剂中的纯RTI-Aav1 I类重链免疫LEW大鼠，得到的反应与移植后所见相同，即CD4⁺ T细胞对肽1有良好增殖，但对肽2和肽3无增殖。然而，用弗氏佐剂中的肽1、2和3免疫LEW大鼠，导致CD4⁺ T细胞对每种肽都有良好的增殖反应。这些数据表明同种异体移植排斥可刺激间接同种异体识别，并强调供体抗原的生理加工将影响哪些肽在移植间接同种异体识别中起重要作用。
