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通过SSR、AFLP和MSAP标记评估的车前草多样性新图谱。

A new image of plantain diversity assessed by SSR, AFLP and MSAP markers.

作者信息

Noyer J L, Causse S, Tomekpe K, Bouet A, Baurens F C

机构信息

CIRAD, UMR 1096 PIA, France.

出版信息

Genetica. 2005 May;124(1):61-9. doi: 10.1007/s10709-004-7319-z.

Abstract

Using both SSR and AFLP markers, the genetic diversity of 30 plantains constituting a representative sample of the phenotypic diversity was assessed. The results confirmed a very narrow genetic base of this cultivar group. SSR and AFLP data support the hypothesis that these cultivars may have arisen from vegetative multiplication of a single seed. MSAP were used to survey cytosine methylation status at CCGG sites in order to obtain an alternative source of diversity data. A higher degree of polymorphism was revealed allowing the classification of the samples into three clusters. No correlation was observed between the phenotypic classification and methylation diversity. Implications for breeding programs are discussed.

摘要

利用简单序列重复(SSR)和扩增片段长度多态性(AFLP)标记,对构成表型多样性代表性样本的30个大蕉的遗传多样性进行了评估。结果证实了该栽培品种组的遗传基础非常狭窄。SSR和AFLP数据支持了这些品种可能源自单一种子营养繁殖的假说。甲基化敏感扩增多态性(MSAP)被用于调查CCGG位点的胞嘧啶甲基化状态,以便获得多样性数据的另一个来源。结果揭示了更高程度的多态性,从而能够将样本分为三个聚类。未观察到表型分类与甲基化多样性之间的相关性。文中讨论了对育种计划的影响。

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