Chao R B, Hu L
School of Pharmacy, West China University of Medical Sciences, Chengdu.
Yao Xue Xue Bao. 1993;28(9):705-8.
A new method for the determination of peimisine in bulbus Fritillariae by ion-pair HPLC has been developed. The chromatographic system consists of ODS column and mobile phase of methanol--water (69:31) containing 7.5 mmol/L of SDS (pH 4.5 +/- 0.1). Twelve samples of different species in the family of Fritillaria have been analyzed by this method. The results show that all samples contain peimisine and the contents of peimisine are correlative to those of total alkaloids.
已开发出一种用离子对高效液相色谱法测定川贝母中浙贝母碱的新方法。色谱系统由ODS柱和甲醇 - 水（69:31）的流动相组成，流动相中含有7.5 mmol/L的十二烷基硫酸钠（pH 4.5±0.1）。用该方法分析了贝母科12个不同物种的样品。结果表明，所有样品均含有浙贝母碱，且浙贝母碱的含量与总生物碱的含量相关。
