沉降场流分离中快速“超层”分离的发展

Fast "hyperlayer" separation development in sedimentation field flow fractionation.

作者信息

Kassab James R, Cardot Philippe J P, Zahoransky Richard A, Battu Serge

机构信息

Laboratoire de Chimie Analytique et de Bromatologie, Université de Limoges, Faculté de Pharmacie, 2 Rue du Dr. Marcland, F-87025 Limoges, Cedex, France.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 5;826(1-2):8-16. doi: 10.1016/j.jchromb.2005.06.020. Epub 2005 Jul 11.

DOI:10.1016/j.jchromb.2005.06.020

PMID:16011912

Abstract

Specific prototypes of sedimentation field flow fractionation devices (SdFFF) have been developed with relative success for cell sorting. However, no data are available to compare these apparatus with commercial ones. In order to compare with other devices mainly used for non-biological species, biocompatible systems were used for standard particle (latex: 3-10 microm of different size dispersities) separation development. In order to enhance size dependent separations, channels of reduced thickness were used (80 and 100 microm) and channel/carrier-phase equilibration procedures were necessary. For sample injection, the use of inlet tubing linked to the FFF accumulation wall, common for cell sorting, can be extended to latex species when they are eluted in the Steric Hyperlayer elution mode. It avoids any primary relaxation steps (stop flow injection procedure) simplifying series of elution processing. Mixtures composed of four different monodispersed latex beads can be eluted in 6 min with 100 microm channel thickness.

摘要

已经开发出特定原型的沉降场流分离装置（SdFFF），在细胞分选方面取得了相对成功。然而，尚无数据可将这些仪器与商业仪器进行比较。为了与主要用于非生物物种的其他装置进行比较，使用生物相容性系统进行标准颗粒（乳胶：3 - 10微米不同尺寸分散度）的分离开发。为了增强尺寸依赖性分离，使用了厚度减小的通道（80和100微米），并且通道/载体相平衡程序是必要的。对于样品注入，连接到FFF积累壁的入口管的使用，这在细胞分选中很常见，当乳胶物种以空间超层洗脱模式洗脱时，可以扩展到乳胶物种。它避免了任何初级弛豫步骤（停流注射程序），简化了一系列洗脱过程。由四种不同单分散乳胶珠组成的混合物可以在6分钟内用100微米通道厚度洗脱。