Jensen K D, Williams S K, Giddings J C
Department of Chemistry, University of Utah, Salt Lake City 84112, USA.
J Chromatogr A. 1996 Oct 4;746(1):137-45. doi: 10.1016/0021-9673(96)00288-9.
The performance of thin (down to 71 microns thick) flow field-flow fractionation (flow FFF) channels was examined at both high channel flow-rates (up to approximately 47 ml/min) and cross flow-rates (up to approximately 11 ml/min). High levels of retention were observed, suggestive of good performance characteristics. Supporting this expectation, four sizes (0.04-0.30 microns) of polystyrene latex microbeads were baseline separated in under 3 min. Nonetheless, a plate height analysis showed that performance was still less than theoretically expected. Fast steric-hyperlayer separations of larger latex beads were observed in the same systems. Furthermore, it was shown that the steric inversion diameter was shifted down to approximately 0.23 micron thus expanding the size range to which this FFF mode is applicable. The steric inversion phenomenon observed using narrow latex standards was shown to be consistent with that found for a polydisperse polyvinylchloride latex.
在高通道流速（高达约47毫升/分钟）和错流流速（高达约11毫升/分钟）下，对薄型（低至71微米厚）的流场-流分级（flow FFF）通道性能进行了检测。观察到了高保留率，表明具有良好的性能特征。四个尺寸（0.04 - 0.30微米）的聚苯乙烯乳胶微珠在3分钟内实现了基线分离，这支持了上述预期。尽管如此，塔板高度分析表明性能仍低于理论预期。在同一系统中观察到了较大乳胶珠的快速空间-超层分离。此外，结果表明空间反演直径下移至约0.23微米，从而扩大了该FFF模式适用的尺寸范围。使用窄乳胶标准品观察到的空间反演现象与多分散聚氯乙烯乳胶的情况一致。
