Chui Chung Hin, Lau Fung Yi, Tang Johnny Cheuk On, Kan Kin Luen, Cheng Gregory Yin Ming, Wong Raymond Siu Ming, Kok Stanton Hon Lung, Lai Paul Bo San, Ho Rocky, Gambari Roberto, Chan Albert Sun Chi
Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hong Kong, P.R. China.
Int J Mol Med. 2005 Aug;16(2):337-41.
The possible antiproliferative and apoptotic inducing potentials of fresh juice prepared from Scutellaria barbata (SBJ) and warmed water extract of Radix Sophorae Tonkinensis (RSTE) have been tested on a series of cancer cell lines, including HepG2 hepatoblastoma, Hep3B hepatocellular carcinoma, MDA-MB231 breast carcinoma, A549 lung cancer and KG-1 acute myelogenous leukaemia in vitro. Both SBJ and RSTE were able to inhibit the growth of cancer cell lines and induce apoptosis. Further analysis of the action of RSTE on HepG2 cells suggested that the activity of the central machinery of apoptosis, caspase 3, was significantly elevated. Oligo-nucleosomal length DNA fragments formation was readily detected by TdT-mediated dUTP nick end labelling assay after RSTE treatment. Taken together, we believe that, although Radix Sophorae Tonkinensis was demonstrated to have toxic components including matrine and oxymatrine, it is still worthwhile to further investigate its anti-cancer potential under a safety toxicological precaution.
半枝莲鲜汁（SBJ）和山豆根温水提取物（RSTE）的潜在抗增殖和诱导凋亡能力已在一系列癌细胞系上进行了体外测试，这些癌细胞系包括HepG2肝母细胞瘤、Hep3B肝细胞癌、MDA - MB231乳腺癌、A549肺癌和KG - 1急性髓性白血病。SBJ和RSTE均能够抑制癌细胞系的生长并诱导凋亡。对RSTE作用于HepG2细胞的进一步分析表明，凋亡核心机制半胱天冬酶3的活性显著升高。RSTE处理后，通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记试验很容易检测到寡核小体长度DNA片段的形成。综上所述，我们认为，虽然山豆根被证明含有包括苦参碱和氧化苦参碱在内的有毒成分，但在安全毒理学预防措施下进一步研究其抗癌潜力仍然是值得的。
