担子菌香菇Le.ras基因启动子区域的特征分析

Characterization of the promoter region of the basidiomycete Lentinus edodes Le.ras gene.

作者信息

Kajiwara S, Shishido K

机构信息

Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

FEMS Microbiol Lett. 1992 Apr 15;71(2):147-50. doi: 10.1016/0378-1097(92)90503-g.

DOI:10.1016/0378-1097(92)90503-g

PMID:1601285

Abstract

An analysis of the 2.7-kb nucleotide sequence including the 5'-flanking region of the basidiomycete Lentinus edodes Le.ras-encoding gene revealed that the promoter region contains a CAAT box, a CACCC box, two consecutive TATAAA boxes, and a CT-rich sequence element, in that order, from upstream to downstream. One major and two alternative transcriptional initiation sites are located 132 nucleotides and 85 and 90 nucleotides downstream from the downstream TATAAA box, and the major site is positioned just in the CT-rich sequence.

摘要

对包括担子菌香菇Le.ras编码基因5'侧翼区在内的2.7 kb核苷酸序列进行分析后发现，启动子区域从上游到下游依次包含一个CAAT盒、一个CACCC盒、两个连续的TATAAA盒和一个富含CT的序列元件。一个主要转录起始位点和两个备选转录起始位点分别位于下游TATAAA盒下游132个核苷酸处以及85和90个核苷酸处，主要位点正好位于富含CT的序列中。