Kondoh O, Shishido K
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
FEMS Microbiol Lett. 1995 Aug 1;130(2-3):189-92. doi: 10.1111/j.1574-6968.1995.tb07718.x.
An analysis of the 2 kb nucleotide sequence including the 5'-flanking region of a cell-adhesion protein-encoding gene (mfbA) isolated from the basidiomycete Lentinus edodes revealed that the promoter region contains a TATA box, a GC box, a CAAT box, a CT-rich sequence element, a TATA box, two CT-rich sequences, and a CAAT box, in the order, from upstream to downstream. Three major and three alternative transcriptional initiation sites were located 127, 129 and 131 nucleotides and 96, 193 and 197 nucleotides downstream from the downstream TATA box, and all the three major sites are positioned just in the most downstream CT-rich sequence. Three 16 bp unique sequences similar to the binding sites of Neurospora crassa transcriptional activator protein qa-1F (Baum et al. (1987) Expression of qa-1F activator protein: Identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain. Mol. Cell. Biol. 7, 1256-1266) were present between the upstream TATA box and upstream CAAT box.
对从担子菌香菇中分离出的编码细胞黏附蛋白的基因（mfbA）的2 kb核苷酸序列（包括5'-侧翼区）进行分析后发现，启动子区域从上游到下游依次包含一个TATA框、一个GC框、一个CAAT框、一个富含CT的序列元件、一个TATA框、两个富含CT的序列和一个CAAT框。三个主要转录起始位点和三个备选转录起始位点分别位于下游TATA框下游127、129和131个核苷酸处以及96、193和197个核苷酸处，并且所有三个主要位点都恰好位于最下游富含CT的序列中。在上游TATA框和上游CAAT框之间存在三个与粗糙脉孢菌转录激活蛋白qa-1F（Baum等人，（1987年）qa-1F激活蛋白的表达：qa基因簇中上游结合位点的鉴定及DNA结合结构域的定位。《分子与细胞生物学》7，1256 - 1266）的结合位点相似的16 bp独特序列。
