Goedkoop Amber Y, de Rie Menno A, Teunissen Marcel B M, Picavet Daisy I, van der Hall Pascal O, Bos Jan D, Tak Paul P, Kraan Maarten C
Department of Dermatology, Academic Medical Center, University of Amsterdam, P.O. Box 22660, 1100 DD Amsterdam, The Netherlands.
Arch Dermatol Res. 2005 Aug;297(2):51-9. doi: 10.1007/s00403-005-0578-4. Epub 2005 Oct 8.
Traditionally the evaluation of the cellular infiltrate and protein expression in skin tissue sections is done by manual quantification. However, for reliable evaluation of histology in the development of new anti-psoriatic treatments there is a need for a more time-efficient and reproducible method. To test the use of digital image analysis (DIA) in this situation we compared the assessment of immunohistochemically stained skin sections with the more traditional manual quantification (MQ) and semi-quantitative analysis (SQA). The number of CD3+ T cells and the expression of E-selectin were evaluated in stained paired skin biopsies from 11 patients with chronic plaque psoriasis before and after initiation of anti-psoriasis therapy. We observed significant correlations between MQ and DIA for the number of T cells (epidermis: r=0.88, P< or =0.01, dermis r=0.87, P< or =0.01). Both DIA and MQ were equally effective in detecting reductions of T-cell numbers in active-treated patients. MQ took 20 h, compared to 6 h for DIA. We also observed significant correlations between SQA and DIA for the expression of E-selectin (r=0.88, P< or =0.01), although DIA was more sensitive than SQA to detect (early) changes. SQA took 10 h, compared to 4 h for DIA. In conclusion, the quantification of the inflammatory infiltrate in psoriatic lesional skin by DIA generated similar results as MQ and SQA in a reliable, reproducible and higher time efficient fashion.
传统上，皮肤组织切片中细胞浸润和蛋白表达的评估是通过手动定量完成的。然而，为了在新的抗银屑病治疗开发中可靠地评估组织学，需要一种更省时且可重复的方法。为了测试在这种情况下数字图像分析（DIA）的应用，我们将免疫组化染色的皮肤切片评估与更传统的手动定量（MQ）和半定量分析（SQA）进行了比较。在11例慢性斑块状银屑病患者开始抗银屑病治疗前后，对配对的皮肤活检染色切片中CD3 + T细胞数量和E-选择素表达进行了评估。我们观察到T细胞数量在MQ和DIA之间存在显著相关性（表皮：r = 0.88，P≤0.01，真皮：r = 0.87，P≤0.01）。在检测接受积极治疗患者的T细胞数量减少方面，DIA和MQ同样有效。MQ需要20小时，而DIA需要6小时。我们还观察到E-选择素表达在SQA和DIA之间存在显著相关性（r = 0.88，P≤0.01），尽管DIA在检测（早期）变化方面比SQA更敏感。SQA需要10小时，而DIA需要4小时。总之，通过DIA对银屑病皮损皮肤中的炎性浸润进行定量，以可靠、可重复且更高时效的方式产生了与MQ和SQA相似的结果。
