Tatapudy Sonali, Bruening Sandra, Gleason Georgia, Toth Miklos
Department of Pharmacology, Weill Cornell Medical College, New York, NY 10021, USA.
J Neurosci Methods. 2008 Jul 30;172(2):173-7. doi: 10.1016/j.jneumeth.2008.04.016. Epub 2008 Apr 25.
The dentate gyrus is one of the few brain regions that show proliferation of neuronal precursors postnatally and in adult life. Proliferation in the dentate gyrus has been shown to be influenced by exercise, stress and drugs such as antidepressants. Traditionally, proliferation studies rely on the time consuming and subjective manual count of labeled cells. Here we adapted the Metamorph software to automatically count cells labeled in the S phase in the developing dentate gyrus of mice. The validity of the computer-assisted method was established by showing an outcome similar to that obtained with the established manual counting procedure. In addition, by using a genetically modified mouse line with increased proliferation, the ability of the computer-assisted method to detect changes in proliferation was demonstrated.
齿状回是少数在出生后及成年期仍显示神经元前体细胞增殖的脑区之一。已表明齿状回中的增殖受运动、应激和抗抑郁药等药物的影响。传统上，增殖研究依赖于对标记细胞进行耗时且主观的手动计数。在此，我们采用了MetaMorph软件来自动计数小鼠发育中的齿状回中处于S期的标记细胞。通过显示与既定手动计数程序获得的结果相似的结果，确立了计算机辅助方法的有效性。此外，通过使用增殖增加的基因改造小鼠品系，证明了计算机辅助方法检测增殖变化的能力。
