Cen Yan, Zhang Ren, Yao Wen-hua, Ma Jiong, Chen Ji-yao
Department of Physics, Fudan University, Shanghai 200433, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Mar;25(3):405-8.
The Raman spectra of nitrosyhemoglobin (NOHb), methemoglobin (MetHb), oxygenated hemoglobin (oxyHb), and deoxygenated hemoglobin(deoxyHb) were comparatively studied. The results show that the Raman spectra with 632.8 nm excitation can be used to detect the R state (oxyHb) to T state (deoxyHb) transition. The difference of NOHb and MetHb in their electron spin state was clearly demonstrated in their Raman spectra with 632.8 nm excitation, suggestingthat Raman spectra provide anew simple way to measure and distinguish NOHb and MetHb.
对亚硝基血红蛋白（NOHb）、高铁血红蛋白（MetHb）、氧合血红蛋白（oxyHb）和脱氧血红蛋白（deoxyHb）的拉曼光谱进行了比较研究。结果表明，632.8nm激发下的拉曼光谱可用于检测R态（氧合血红蛋白）到T态（脱氧血红蛋白）的转变。在632.8nm激发的拉曼光谱中，NOHb和MetHb在电子自旋状态上的差异得到了清晰的展示，这表明拉曼光谱为测量和区分NOHb和MetHb提供了一种新的简单方法。
