人血红蛋白衍生物的测定

Determination of Human Hemoglobin Derivatives.

作者信息

Attia Atef M M, Ibrahim Fatma A A, Abd El-Latif Noha A, Aziz Samir W, Abdelmottaleb Moussa Sherif A, Elalfy Mohsen S

机构信息

a Biophysical Laboratory, Biochemistry Department , National Research Centre , Dokki , Cairo , Egypt.

出版信息

Hemoglobin. 2015;39(5):371-4. doi: 10.3109/03630269.2015.1062775. Epub 2015 Jul 21.

DOI:10.3109/03630269.2015.1062775

PMID:26193973

Abstract

The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%) and oxyHb% (97.06-98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608-15.777 g/dL). The method is highly sensitive, accurate and reproducible.

摘要

采用新开发的多组分分光光度法测定了健康未孕女性志愿者体内非活性血红蛋白（Hb）色素[如高铁血红蛋白（metHb）、碳氧血红蛋白（HbCO）和硫血红蛋白（SHb）]以及活性Hb[氧合血红蛋白（oxyHb）形式]的水平，以及血液Hb浓度。该方法的结果显示，SHb%的值在（0.0727 - 0.370%）范围内，metHb%（0.43 - 1.0%），HbCO%（0.4 - 1.52%），oxyHb%（97.06 - 98.62%）。此外，该方法的结果显示血液Hb浓度的值在（12.608 - 15.777 g/dL）范围内。该方法具有高灵敏度、准确性和可重复性。