Rodríguez-Lázaro David, Jofré Anna, Aymerich Teresa, Garriga Margarita, Pla Maria
Institute of Food and Agricultural Technology (INTEA), University of Girona, Campus Montilivi (edif. Politècnica1), E-17071 Girona, Spain.
J Food Prot. 2005 Jul;68(7):1467-71. doi: 10.4315/0362-028x-68.7.1467.
The spread and persistence of Listeria monocytogenes in smoked fish products and seafood processing factories are big concerns. Thus, the corresponding quality assurance programs must include adequate microbiological control measures. We evaluated eight different pre-PCR sample processing strategies to be coupled with a previously developed real-time PCR assay for the quantitative detection of L. monocytogenes in salmon products. The optimal pre-PCR procedure involved filtration and DNA purification with the use of a commercial kit. This strategy could detect 10 CFU of L. monocytogenes per g of smoked salmon and could quantify 1,000 CFU/g with excellent accuracy compared with the standard plate count method. Thus, this method could be a promising alternative for the quantitative detection of L. monocytogenes in smoked fish products and processing factories. This method could also detect the bacterium in raw salmon.
单核细胞增生李斯特菌在烟熏鱼产品和海鲜加工厂中的传播及持续存在是重大问题。因此，相应的质量保证计划必须包括适当的微生物控制措施。我们评估了八种不同的PCR前样品处理策略，并将其与先前开发的用于定量检测鲑鱼产品中单核细胞增生李斯特菌的实时PCR检测方法相结合。最佳的PCR前程序包括使用商业试剂盒进行过滤和DNA纯化。与标准平板计数法相比，该策略能够检测出每克烟熏三文鱼中10 CFU的单核细胞增生李斯特菌，并且能够以极高的准确度对1000 CFU/g进行定量。因此，该方法有望成为烟熏鱼产品和加工厂中单核细胞增生李斯特菌定量检测的替代方法。该方法还能够检测生三文鱼中的这种细菌。
