应用一步实时聚合酶链反应法检测即食食品中的单核细胞增生李斯特菌。
Detection of Listeria monocytogenes in ready-to-eat food by Step One real-time polymerase chain reaction.
机构信息
Department of Microbiology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
出版信息
J Environ Sci Health B. 2012;47(3):212-6. doi: 10.1080/03601234.2012.634353.
The aim of this study was to follow contamination of ready-to-eat food with Listeria monocytogenes by using the Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. In 30 samples of ready-to-eat milk and meat products without incubation we detected strains of Listeria monocytogenes in five samples (swabs). Internal positive control (IPC) was positive in all samples. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in ready-to-eat food without incubation.
本研究旨在通过使用一步实时聚合酶链反应（PCR）来跟踪即食食品中单核细胞增生李斯特菌的污染情况。我们使用 PrepSEQ Rapid Spin 样品制备试剂盒分离 DNA，并使用 MicroSEQ®单核细胞增生李斯特菌检测试剂盒进行实时 PCR 检测。在对 30 份未经孵育的即食牛奶和肉类产品的检测中，我们在五个样本（拭子）中检测到了单核细胞增生李斯特菌。所有样本的内部阳性对照（IPC）均为阳性。研究结果表明，本研究中开发的实时 PCR 检测方法可灵敏地检测未经孵育的即食食品中的单核细胞增生李斯特菌。
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