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[伯氏疏螺旋体抗体阳性患者(无症状携带者)的维生素A、E和C血清浓度]

[Vitamin A, E and C serum concentration in patients with Borrelia burgdorferi antibodies--non-symptomatic carriers].

作者信息

Pancewicz Sławomir A, Skrzydlewska Elzbieta, Hermanowska-Szpakowicz Teresa, Stankiewicz Anna, Sniecińska Anna, Kondrusik Maciej, Zajkowska Joanna, Swierzbińska Renata

机构信息

Klinika Chorób Zakaznych i Neuroinfekcji AMB.

出版信息

Przegl Epidemiol. 2005;59(1):35-41.

PMID:16013408
Abstract

To estimate vitamin A, E and C serum concentrations among forestry workers showing antibodies against Borrelia burgdorferi presence. Vitamins A, E and C concentrations were evaluated in 117 sera of forestry workers. 78 persons aged 18-63 (x=43.07) showed antibodies against Borrelia burgdorferi presence. In this group 13 persons showed presence of IgM, 42 persons with IgG and 23 with IgM and IgG. Control group consisted of 39 persons aged 18-56 years (x=40,97), with no detectable anti-Borrelia burgdorferi antibodies in serum. Serologic diagnosis was performed with use of ELISA kit - Borrelia recombinant IgM, IgG (Biomedica, Austria). Vitamins A and E serum concentrations were detected by RP-HPLC method with spectrophotometric detection (De Leenheet and co.). Vitamin C was detected by RP-HPLS method with spectrophotometric method (Ivanovic and co). Obtains results were statistically analysed. Significantly lower of vitamin A and E serum concentration of persons with anti-borrelia antibodies presence. The lowest concentration was observed in group showing presence of IgM and IgG. No significant difference in vitamin C serum concentration in examined groups was observed. These results may suggest that low serum concentrations of vitamin A and E may have influence on Borrelia burgdorferi infection development.

摘要

评估显示存在抗伯氏疏螺旋体抗体的林业工人血清中维生素A、E和C的浓度。对117名林业工人的血清进行了维生素A、E和C浓度评估。78名年龄在18 - 63岁(x = 43.07)的人显示存在抗伯氏疏螺旋体抗体。在该组中,13人显示IgM阳性,42人显示IgG阳性,23人显示IgM和IgG均阳性。对照组由39名年龄在18 - 56岁(x = 40.97)的人组成,其血清中未检测到抗伯氏疏螺旋体抗体。使用ELISA试剂盒 - 伯氏疏螺旋体重组IgM、IgG(奥地利Biomedica公司)进行血清学诊断。采用反相高效液相色谱法(RP - HPLC)结合分光光度检测法(De Leenheet等人)检测血清中维生素A和E的浓度。采用反相高效液相色谱法(RP - HPLS)结合分光光度法(Ivanovic等人)检测维生素C。对所得结果进行统计学分析。存在抗伯氏疏螺旋体抗体的人的血清中维生素A和E浓度显著较低。在显示IgM和IgG均阳性的组中观察到最低浓度。在所检查的组中未观察到维生素C血清浓度有显著差异。这些结果可能表明血清中维生素A和E浓度较低可能对伯氏疏螺旋体感染的发展有影响。

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[Vitamin A, E and C serum concentration in patients with Borrelia burgdorferi antibodies--non-symptomatic carriers].[伯氏疏螺旋体抗体阳性患者(无症状携带者)的维生素A、E和C血清浓度]
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