Chmielewska-Badora Jolanta, Moniuszko Anna, Żukiewicz-Sobczak Wioletta, Zwoliński Jacek, Piątek Jacek, Pancewicz Sławomir
Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland.
Ann Agric Environ Med. 2012;19(2):271-4.
Sera of 39 farmers, 119 foresters and 32 blood donors were investigated for the presence of antibodies against B. burgdorferi, A. phagocytophilum, B. microti and Bartonella spp. Semi-quantitative indirect immunofluorescence test was used to measure titers of anti-A. phagocytophilum, B. microti and Bartonella spp. IgG. ELISA test was used to measure titers of anti-B. burgdorferi IgM and IgG. B. burgdorferi was the most frequently observed among all the examined pathogens. 27.7% of farmers, 23.1% of forestry workers and 37.5% of control group were infected with Bartonella spp. Anti-A. phagocytophilum and anti-B. microti reactions were observed rarely. Sera of persons with single infection dominated in farmers and forestry workers. Co-infection with 2 pathogens was observed more frequently in forestry workers and farmers than in the control group. Co-infections with 3-4 pathogens were observed only in forestry workers. Among the observed co-infections, the most frequent were: B. burgdorferi with Bartonella spp. and B. burgdorferi with A. phagocytophilum. Moreover, in forestry workers, triple coinfections with B. burgdorferi, Bartonella spp. and A. phagocytophilum and one quadruple coinfection were observed. Persons with occupational risk of tick bites, especially forestry workers, more often have anti-B. burgdorferi antibodies and are more often co-infected with various tick-borne pathogens than the persons from the control group. It seems that more often coinfections in persons with occupational risk of tick bites are a consequence of the higher incidence of infection with B. burgdorferi, as anti-B. microti, A. phagocytophilum and Bartonella spp. antibodies are not more commonly prevalent in persons with occupational risk of tick bites than in healthy volunteers.
对39名农民、119名林业工人和32名献血者的血清进行了检测，以确定是否存在抗伯氏疏螺旋体、嗜吞噬细胞无形体、微小巴贝斯虫和巴尔通体属的抗体。采用半定量间接免疫荧光试验检测抗嗜吞噬细胞无形体、微小巴贝斯虫和巴尔通体属IgG的滴度。采用酶联免疫吸附测定（ELISA）试验检测抗伯氏疏螺旋体IgM和IgG的滴度。在所有检测的病原体中，伯氏疏螺旋体是最常观察到的。27.7%的农民、23.1%的林业工人和37.5%的对照组感染了巴尔通体属。抗嗜吞噬细胞无形体和抗微小巴贝斯虫反应很少见。农民和林业工人中以单一感染的人的血清为主。林业工人和农民中两种病原体的合并感染比对照组更常见。仅在林业工人中观察到3 - 4种病原体的合并感染。在观察到的合并感染中，最常见的是：伯氏疏螺旋体与巴尔通体属以及伯氏疏螺旋体与嗜吞噬细胞无形体。此外，在林业工人中，观察到伯氏疏螺旋体、巴尔通体属和嗜吞噬细胞无形体的三重合并感染以及一例四重合并感染。有蜱叮咬职业风险的人，尤其是林业工人，比对照组的人更常拥有抗伯氏疏螺旋体抗体，并且更常感染各种蜱传病原体。似乎有蜱叮咬职业风险的人更常出现合并感染是伯氏疏螺旋体感染发生率较高的结果，因为抗微小巴贝斯虫、嗜吞噬细胞无形体和巴尔通体属抗体在有蜱叮咬职业风险的人中并不比健康志愿者更普遍。
