系统性硬化症患者皮肤中T细胞的早期激活。
Early T cell activation in the skin from patients with systemic sclerosis.
作者信息
Kalogerou A, Gelou E, Mountantonakis S, Settas L, Zafiriou E, Sakkas L
机构信息
Department of Medicine/Rheumatology, Thessaly University School of Medicine, Larisa, Greece.
出版信息
Ann Rheum Dis. 2005 Aug;64(8):1233-5. doi: 10.1136/ard.2004.027094.
OBJECTIVES
To determine if T cells in skin lesions in systemic sclerosis (SSc) express the early activation antigen CD69 that participates in cell-cell interactions.
METHODS
Skin biopsy specimens from 17 patients with SSc were analysed by immunohistochemistry using the indirect peroxidase method and monoclonal antibodies for CD3 (T cell marker), CD69 (early T cell activation marker), and CD68 (macrophage marker).
RESULTS
Mononuclear cells, containing mostly T cells and macrophages, were increased in SSc skin lesions and were present in perivascular areas. CD69 was expressed in these mononuclear cells. There was no correlation between the number of CD3+, CD69+, or CD68+ cells and the Rodnan skin score or disease duration.
CONCLUSIONS
The expression of early T cell activation antigen CD69 in skin lesions suggests that T cells may actively participate in cell-cell contact with fibroblasts to promote fibrosis.
目的
确定系统性硬化症（SSc）皮肤病变中的T细胞是否表达参与细胞间相互作用的早期激活抗原CD69。
方法
采用间接过氧化物酶法和针对CD3（T细胞标志物）、CD69（早期T细胞激活标志物）及CD68（巨噬细胞标志物）的单克隆抗体，通过免疫组织化学分析17例SSc患者的皮肤活检标本。
结果
SSc皮肤病变中单核细胞增多，主要为T细胞和巨噬细胞，且存在于血管周围区域。这些单核细胞表达CD69。CD3⁺、CD69⁺或CD68⁺细胞数量与罗德南皮肤评分或病程之间无相关性。
结论
皮肤病变中早期T细胞激活抗原CD69的表达提示T细胞可能积极参与与成纤维细胞的细胞间接触以促进纤维化。
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