Martínez Clara, Ibarra Jorge E, Caballero Primitivo
Laboratorio de Entomología Agrícola y Patología de Insectos, Departamento de Producción Agraria, Universidad Pública de Navarra, 31006 Pamplona, Spain.
J Invertebr Pathol. 2005 Oct;90(2):91-7. doi: 10.1016/j.jip.2005.05.003. Epub 2005 Jul 12.
Serotyping, cry gene content, and toxicity to Helicoverpa armigera were determined for 178 isolates of Bacillus thuringiensis native to Spain. A total of 13 different cry1 and cry2 genes were detected when isolates were screened by PCR analysis. Results showed that cry2 and cry1Ia were the most frequent cry genes in the collection (74 and 57%, respectively); whereas cry1D, cry1Aa, cry1Ab, and cry1C were only moderately abundant (49, 48, 47, and 36%, respectively). The most uncommon cry genes were cry1Ac, cry1E, cry1B, cry1Ib, cry1Ad, cry1F, and cry1G, with frequencies of 24, 14, 13, 8, 5, 5, and 1%, respectively. The distribution of some cry genes was somewhat associated with particular serovars. For example, genes cry1C and cry1D were especially frequent in the serovar aizawai, while cry1B was very frequent in the serovar thuringiensis. Bioassays against H. armigera larvae showed a wide variation in the insecticidal potency, even among strains sharing the same set of cry genes and within the same serotype.
对来自西班牙的178株苏云金芽孢杆菌分离株进行了血清型鉴定、cry基因含量测定以及对棉铃虫的毒性测定。通过PCR分析筛选分离株时，共检测到13种不同的cry1和cry2基因。结果表明，cry2和cry1Ia是该菌库中最常见的cry基因（分别为74%和57%）；而cry1D、cry1Aa、cry1Ab和cry1C的丰度仅为中等水平（分别为49%、48%、47%和36%）。最不常见的cry基因是cry1Ac、cry1E、cry1B、cry1Ib、cry1Ad、cry1F和cry1G，其频率分别为24%、14%、13%、8%、5%、5%和1%。一些cry基因的分布与特定血清型有一定关联。例如，cry1C和cry1D基因在aizawai血清型中尤为常见，而cry1B在thuringiensis血清型中非常常见。对棉铃虫幼虫的生物测定表明，即使在具有相同cry基因组合的菌株之间以及同一血清型内，杀虫效力也存在很大差异。
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