Chen Fu-Chu, Tsai Ming-Chu, Peng Cheng-Hung, Chak Kin-Fu
Institute of Biochemistry, National Yang Ming University, Peitou, Taipei 11221, Taiwan.
Curr Microbiol. 2004 Apr;48(4):270-5. doi: 10.1007/s00284-003-4195-1.
On the basis of the newly revised nomenclature system of cry genes, the PCR amplification method has been adopted to resolve the cry gene combinations of 294 Bacillus thuringiensis isolates from five selected areas of Taiwan. Our results indicate that cry1 (especially cry1A + 1B + 1F) and cry2 were the most abundant cry genes in Taiwan. In contrast, cry3 and cry6 genes were detected only on Yang Ming Mountain, while the cry13 gene was found only on Snow Mountain. In addition, some distinctive combinations of cry genes were detected in distinct areas of Taiwan, such as cry1C, cry1D, cry1C + 1D, cry4, cry1 + 4, cry1 + 11, cry4 + 11, and cry1 + 4 + 11 in the Taipei area; cry1A + 1C + 1F in the Taichung area; cry1E and cry1A + 1B + 1I on Yang Ming Mountain; cry1 + 13, cry1 + 2 + 11, and cry1 + 2 + 13 on Snow Mountain; and cry1 + 5 and cry1 + 2 + 5 on Jade Mountain. These data clearly indicate that the distribution of cry gene combinations of B. thuringiensis isolates seems to be geographically related.
基于苏云金芽孢杆菌cry基因新修订的命名系统，采用聚合酶链式反应（PCR）扩增方法解析了来自台湾五个选定区域的294株苏云金芽孢杆菌分离株的cry基因组合。我们的结果表明，cry1（尤其是cry1A + 1B + 1F）和cry2是台湾地区最丰富的cry基因。相比之下，cry3和cry6基因仅在阳明山被检测到，而cry13基因仅在雪山被发现。此外，在台湾不同地区检测到了一些独特的cry基因组合，例如台北地区的cry1C, cry1D, cry1C + 1D, cry4, cry1 + 4, cry1 + 11, cry4 + 11和cry1 + 4 + 11；台中地区的cry1A + 1C + 1F；阳明山的cry1E和cry1A + 1B + 1I；雪山的cry1 + 13, cry1 + 2 + 11和cry1 + 2 + 13；以及玉山的cry1 + 5和cry1 + 2 + 5。这些数据清楚地表明，苏云金芽孢杆菌分离株的cry基因组合分布似乎与地理位置有关。
