Genovese Tiziana, Mazzon Emanuela, Rossi Antonietta, Di Paola Rosanna, Cannavò Giuseppe, Muià Carmelo, Crisafulli Concetta, Bramanti Placido, Sautebin Lidia, Cuzzocrea Salvatore
Dipartimento Clinico Sperimentale di Medicina e Farmacologia, Facoltà di Medicina e Chirurgia, Università di Messina, Italy.
J Neuroimmunol. 2005 Sep;166(1-2):55-64. doi: 10.1016/j.jneuroim.2005.05.009.
A traumatic spinal cord injury (SCI) induces a sequelae of events which conduce biochemical and cellular alterations. Here we compare the degree of spinal cord injury caused by the application of vascular clips, in mice lacking the 5-lipoxygenase and in the corresponding wild-type mice. Biochemical, immunohistochemical and functional studies revealed respectively an increase of neutrophils infiltration, of IL-1beta, TNF-alpha immunoreactivity, apoptosis (measured by Annexin-V staining) and loss of hind legs movement in SCI operated 5-LO wild-type mice. In contrast, the degree of (1) neutrophil infiltration at different time points, (2) cytokine expression (TNF-alpha and IL-1beta), (3) histological damage, (4) apoptosis, was markedly reduced in the tissues obtained from SCI operated 5-LO deficient mice and (5) the motor recovery was ameliorated.
创伤性脊髓损伤（SCI）会引发一系列导致生化和细胞改变的事件。在此，我们比较了在缺乏5-脂氧合酶的小鼠和相应野生型小鼠中，应用血管夹所造成的脊髓损伤程度。生化、免疫组织化学和功能研究分别显示，在接受SCI手术的5-脂氧合酶野生型小鼠中，中性粒细胞浸润增加、IL-1β和TNF-α免疫反应性增强、细胞凋亡（通过膜联蛋白-V染色测定）以及后肢运动丧失。相比之下，在接受SCI手术的5-脂氧合酶缺陷小鼠的组织中，（1）不同时间点的中性粒细胞浸润程度、（2）细胞因子表达（TNF-α和IL-1β）、（3）组织学损伤、（4）细胞凋亡均显著降低，并且（5）运动恢复得到改善。
