Li Yangqiu, Chen Shaohua, Yang Lijian, Zhou Yubing, Wu Xiuli, Huang Meijuan, Geng Suxia
Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
Hematology. 2005 Apr;10(2):135-9. doi: 10.1080/10245330400026196.
T-cell receptor Vss gene repertoire and clonality have been studied in patients with leukemia and solid tumors, by assaying the CDR3 size of TCR genes, using RT-PCR and genescan analysis. Few studies have studied leukemia-associated oligoclonal expanded T-cells in leukemia, therefore, the aim of this study was to investigate the distribution and clonal expansion of T-cell receptor, Vss subfamily T-cells in patients with acute promyelocytic leukemia (APL) with t(15;17). The CDR3 of TCR Vbeta24 subfamily genes were analyzed in peripheral blood mononuclear cells from 17 cases with PML-RARalpha+ APL using RT-PCR and genescan technique. Ten normal individuals served as controls. The results showed that the number of expressed Vss subfamilies (from 2 to 21 subfamilies) varied in different patients with APL. The most frequently expressed Vss subfamilies were Vbeta2 (64.7%), Vbeta15 (58.8%), Vbeta3 and Vbeta5 (47.1%), with a lower expression rate found in Vbeta11 and Vbeta20 (11.7%). Clonally expanded T-cells in the Vss subfamilies could be identified in patients with APL in all but two of the cases studied, predominantly in Vbeta10, Vbeta23, Vbeta3 and Vbeta21. In conclusion, skewed distribution and clonal expansion of TCR Vss subfamily T-cells could be found in patients with APL. The clonal expansion of T-cells were considered to be a specific anti-leukemic immune response by host T-cells activated by the leukemia-associated-antigen.
通过逆转录聚合酶链反应（RT-PCR）和基因扫描分析来检测T细胞受体Vβ基因库和克隆性，已在白血病和实体瘤患者中开展了相关研究。然而，针对白血病相关寡克隆性扩增T细胞在白血病中的研究较少。因此，本研究旨在调查伴有t(15;17)的急性早幼粒细胞白血病（APL）患者中T细胞受体Vβ亚家族T细胞的分布及克隆性扩增情况。运用RT-PCR和基因扫描技术，对17例PML-RARα+ APL患者外周血单个核细胞中的TCR Vβ24亚家族基因的互补决定区3（CDR3）进行了分析。选取10名正常个体作为对照。结果显示，不同APL患者中表达的Vβ亚家族数量（从2个到21个亚家族）存在差异。最常表达的Vβ亚家族为Vβ2（64.7%）、Vβ15（58.8%）、Vβ3和Vβ5（47.1%），而Vβ11和Vβ20的表达率较低（11.7%）。在所研究的病例中，除两例之外，其余APL患者均可检测到Vβ亚家族中克隆性扩增的T细胞，主要集中在Vβ10、Vβ23、Vβ3和Vβ21。综上所述，APL患者中可发现TCR Vβ亚家族T细胞的分布偏移及克隆性扩增。T细胞的克隆性扩增被认为是由白血病相关抗原激活的宿主T细胞产生的特异性抗白血病免疫反应。
