McKenna Kyle C, Anderson Kimberly M, Kapp Judith A
Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA, USA.
Ocul Immunol Inflamm. 2005 Apr-Jun;13(2-3):149-57. doi: 10.1080/09273940590933520.
We tested whether antigen administration via the anterior chamber (a.c.) was equivalent to intravenous (i.v.) or mucosal administration antigen.
Ovalbumin (OVA)-specific CD8(+) T cells (OT-I) were enumerated in lymphoid tissues of C57Bl/6 (B6) mice via adoptive transfer after the same amount of antigen was administered via a.c., i.v., or mucosal routes. Lytic activity was measured in B6 and gammadeltaT cell-deficient B6 mice given OVA via a.c., i.v, or mucosal routes after injection with OVA in adjuvant.
OVA a.c. induced a pattern of T-cell proliferation distinct from i.v. or mucosal administration. A.c. and i.v., but not mucosal, OVA induced cytolytic T lymphocyte (CTL) tolerance. The inhibition of CTL responses was significantly greater in mice given OVA a.c. rather than i.v. gammadeltaT cells contributed to a.c.-, but not i.v.-, induced CTL tolerance.
A.c. administration of antigen not de-facto i.v. or mucosal administration of antigen.
我们测试了通过前房（a.c.）给予抗原是否等同于静脉内（i.v.）或黏膜给予抗原。
在通过前房、静脉内或黏膜途径给予等量抗原后，通过过继转移在C57Bl/6（B6）小鼠的淋巴组织中计数卵清蛋白（OVA）特异性CD8(+) T细胞（OT-I）。在佐剂中注射OVA后，通过前房、静脉内或黏膜途径给予OVA的B6和γδT细胞缺陷型B6小鼠中测量裂解活性。
OVA前房给予诱导了与静脉内或黏膜给予不同的T细胞增殖模式。前房和静脉内给予OVA（而非黏膜给予）诱导了细胞毒性T淋巴细胞（CTL）耐受。在前房给予OVA的小鼠中，CTL反应的抑制明显大于静脉内给予。γδT细胞促成了前房给予（而非静脉内给予）诱导的CTL耐受。
前房给予抗原并非事实上的静脉内或黏膜给予抗原。
