通过高效液相色谱-荧光检测法对长春瑞滨进行生物分析。

Bio-analysis of vinorelbine by high-performance liquid chromatography with fluorescence detection.

作者信息

van Tellingen O, Kuijpers A, Beijnen J H, Baselier M R, Burghouts J T, Nooyen W J

机构信息

Department of Clinical Chemistry, Netherlands Cancer Institute, Antoni van Leeuwenhoekhuis (AvL), Amsterdam.

出版信息

J Chromatogr. 1992 Jan 17;573(2):328-32. doi: 10.1016/0378-4347(92)80138-g.

DOI:10.1016/0378-4347(92)80138-g

PMID:1601969

Abstract

A simple and selective procedure for the determination of vinorelbine, a new semi-synthetic vinca alkaloid, is presented. The method is based on ion-exchange high-performance liquid chromatography on normal-phase silica with fluorescence detection, combined with liquid-liquid extraction using diethyl ether for sample clean-up. The absence of endogenous interferences and the excellent chromatographic behaviour of vinca alkaloids provides accurate results even at low concentrations. The limit of determination in plasma is 1.5 micrograms/l (500-microliters sample). Reproducible recoveries in urine were obtained if 10-50 microliters of sample were processed supplemented with 500 microliters of blank plasma.

摘要

本文介绍了一种测定新型半合成长春花生物碱长春瑞滨的简单且选择性的方法。该方法基于正相硅胶上的离子交换高效液相色谱法，并采用荧光检测，同时结合使用乙醚进行液 - 液萃取以净化样品。内源性干扰的不存在以及长春花生物碱出色的色谱行为，即使在低浓度下也能提供准确的结果。血浆中的测定限为1.5微克/升（500微升样品）。如果对10 - 50微升样品补充500微升空白血浆进行处理，尿液中的回收率具有可重复性。