采用直接进样高效液相色谱法测定血清中的土霉素。

Determination of oxytetracycline in blood serum by high-performance liquid chromatography with direct injection.

作者信息

Ueno R, Uno K, Aoki T

机构信息

Faculty of Bioresources, Mie University, Japan.

出版信息

J Chromatogr. 1992 Jan 17;573(2):333-5. doi: 10.1016/0378-4347(92)80139-h.

DOI:10.1016/0378-4347(92)80139-h

PMID:1601970

Abstract

A direct injection analysis by high-performance liquid chromatography has been developed for oxytetracycline in serum of animals and fish. A Hisep shielded hydrophobic phase column (15 cm x 4.6 mm I.D.) and a mobile phase of methanol-0.2 M oxalic acid (10:90, v/v, pH 7.0) with ultraviolet detection at 360 nm were used. The standard calibration curves in serum of chicken, hog, cattle and rainbow trout were linear over the range 0.1-20 micrograms/ml. The recoveries of oxytetracycline from all serum samples determined at two different concentrations (0.5 and 2.0 micrograms/ml) were 88-103%. The detection limit was 0.05 micrograms/ml for every serum sample.

摘要

已开发出一种用于动物和鱼类血清中土霉素的高效液相色谱直接进样分析方法。使用Hisep屏蔽疏水相柱（15 cm×4.6 mm内径），流动相为甲醇 - 0.2 M草酸（10:90，v/v，pH 7.0），在360 nm处进行紫外检测。鸡、猪、牛和虹鳟鱼血清中的标准校准曲线在0.1 - 20微克/毫升范围内呈线性。在两种不同浓度（0.5和2.0微克/毫升）下测定的所有血清样品中土霉素的回收率为88 - 103%。每个血清样品的检测限为0.05微克/毫升。