Uno K, Maeda I
Department of Food Science, Konan Women's College, Aichi, Japan.
J Chromatogr B Biomed Appl. 1995 Jan 6;663(1):177-80. doi: 10.1016/0378-4347(94)00412-x.
A high-performance liquid chromatographic method with direct injection has been developed for the simultaneous determination of sulphamonomethoxine and its N4-acetyl metabolite in serum of animals and fish. A HISEP shielded hydrophobic-phase column (15 cm x 4.6 mm I.D.), a mobile phase of 0.05 M citric acid-0.2 M disodium hydrogenphosphate-acetonitrile (70:15:15, v/v), and ultraviolet detection at 265 nm were used. The standard calibration curves in serum of chicken, pig, cattle, rainbow trout and yellowtail were linear over the range 0.5-20 micrograms/ml. The recoveries of sulphamonomethoxine and its N4-acetyl metabolite from all serum samples determined at different concentrations (0.5, 2.0 and 10.0 micrograms/ml) were 93-103% and 90-103%, respectively. The lowest measurable sulphamonomethoxine and N4-acetyl metabolite concentrations were 0.04 and 0.1 microgram/ml, respectively, for all serum samples.
已开发出一种直接进样的高效液相色谱法，用于同时测定动物和鱼类血清中的磺胺间甲氧嘧啶及其N4-乙酰代谢物。使用HISEP屏蔽疏水相柱（15 cm×4.6 mm内径）、0.05 M柠檬酸-0.2 M磷酸氢二钠-乙腈（70:15:15，v/v）的流动相，并在265 nm处进行紫外检测。鸡、猪、牛、虹鳟鱼和黄尾鱼血清中的标准校准曲线在0.5-20微克/毫升范围内呈线性。在不同浓度（0.5、2.0和10.0微克/毫升）下测定的所有血清样品中，磺胺间甲氧嘧啶及其N4-乙酰代谢物的回收率分别为93-103%和90-103%。所有血清样品中，磺胺间甲氧嘧啶和N4-乙酰代谢物的最低可测浓度分别为0.04和0.1微克/毫升。
