Devoto Alessandra, Ellis Christine, Magusin Andreas, Chang Hur-Song, Chilcott Charles, Zhu Tong, Turner John G
School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK.
Plant Mol Biol. 2005 Jul;58(4):497-513. doi: 10.1007/s11103-005-7306-5.
The Arabidopsis gene COI1 is required for jasmonic acid (JA)-induced growth inhibition, resistance to insect herbivory, and resistance to pathogens. In addition, COI1 is also required for transcription of several genes induced by wounding or by JA. Here, we use microarray gene transcription profiling of wild type and coi1 mutant plants to examine the extent of the requirement of COI1 for JA-induced and wound-induced gene transcription. We show that COI1 is required for expression of approximately 84% of 212 genes induced by JA, and for expression of approximately 44% of 153 genes induced by wounding. Surprisingly, COI1 was also required for repression of 53% of 104 genes whose expression was suppressed by JA, and for repression of approximately 46% of 83 genes whose expression was suppressed by wounding. These results indicate that COI1 plays a pivotal role in wound- and JA signalling.
拟南芥基因COI1对于茉莉酸（JA）诱导的生长抑制、对昆虫食草作用的抗性以及对病原体的抗性是必需的。此外，COI1对于由创伤或JA诱导的几个基因的转录也是必需的。在这里，我们使用野生型和coi1突变体植物的微阵列基因转录谱来检查COI1对JA诱导和创伤诱导的基因转录的需求程度。我们表明，COI1对于JA诱导的212个基因中约84%的表达是必需的，对于创伤诱导的153个基因中约44%的表达是必需的。令人惊讶的是，COI1对于JA抑制表达的104个基因中53%的抑制也是必需的，对于创伤抑制表达的83个基因中约46%的抑制也是必需的。这些结果表明，COI1在创伤和JA信号传导中起关键作用。
