Zhang Xiaohong, Feng Baomin, Zhang Qing, Zhang Diya, Altman Naomi, Ma Hong
Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 405D Life Sciences Building, University Park, PA 16802, USA.
Plant Mol Biol. 2005 Jun;58(3):401-19. doi: 10.1007/s11103-005-5434-6.
We have used oligonucleotide microarrays to detect Arabidopsis gene expression during early flower development. Among the 22,746 genes represented on the Affymetrix ATH1 chip, approximately 14,660 (approximately 64.5%) genes were expressed with signal intensity at or more than 50 in each of the six organs/structures examined, including young inflorescences (floral stages 1-9), stage-12 floral buds, developing siliques, leaves, stems, and roots. 17,583 genes were expressed with an intensity at or above 50 in at least one tissue, including 12,245 genes that were expressed in all the six tissues. Comparison of genes expressed between young inflorescence or stage-12 floral buds with other tissues suggests that relatively large numbers of genes are expressed at similar levels in tissues that are related morphologically and/or developmentally, as supported by a cluster analysis with data from two other studies. Further analysis of the genes preferentially expressed in floral tissues has uncovered new genes potentially involved in Arabidopsis flower development. One hundred and four genes were determined to be preferentially expressed in young inflorescences, including 22 genes encoding putative transcription factors. We also identified 105 genes that were preferentially expressed in three reproductive structures (the young inflorescences, stage-12 floral buds and developing siliques), when compared with the vegetative tissues. RT-PCR results of selected genes are consistent with the results from these microarrays and suggest that the relative signal intensities detected with the Affymetrix microarray are reliable estimates of gene expression.
我们利用寡核苷酸微阵列检测拟南芥早期花发育过程中的基因表达。在Affymetrix ATH1芯片上所代表的22746个基因中，在所检测的六个器官/结构（包括幼嫩花序（花发育阶段1 - 9）、12期花芽、发育中的角果、叶片、茎和根）中，约14660个（约64.5%）基因表达，其信号强度达到或超过50。17583个基因在至少一个组织中表达强度达到或高于50，其中12245个基因在所有六个组织中均有表达。对幼嫩花序或12期花芽与其他组织之间表达的基因进行比较表明，形态学和/或发育上相关的组织中，相对大量的基因以相似水平表达，另外两项研究的数据进行的聚类分析也支持这一点。对在花组织中优先表达的基因进行进一步分析，发现了可能参与拟南芥花发育的新基因。确定有104个基因在幼嫩花序中优先表达，其中包括22个编码假定转录因子的基因。与营养组织相比，我们还鉴定出105个在三种生殖结构（幼嫩花序、12期花芽和发育中的角果）中优先表达的基因。所选基因的RT-PCR结果与这些微阵列的结果一致，表明用Affymetrix微阵列检测到的相对信号强度是基因表达的可靠估计。
