Lee Jeong-Won, Yong Song Sang, Choi Jung-Joo, Lee Sun-Joo, Kim Byoung-Gie, Park Chang-Soo, Lee Je-Ho, Lin Chen-Yong, Dickson Robert B, Bae Duk-Soo
Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Hum Pathol. 2005 Jun;36(6):626-33. doi: 10.1016/j.humpath.2005.03.003.
Matriptase is an epithelial-derived, integral serine protease that has been implicated in the progression of epithelial tumors. We investigated whether the expression of matriptase is associated with the progression of cervical neoplasia. Using immunohistochemistry, we evaluated the matriptase expression in 89 formalin-fixed paraffin-embedded cervical tissues that included 10 normal cervical specimens, 19 low-grade squamous intraepithelial lesions, 20 high-grade squamous intraepithelial lesions, 20 invasive squamous cell carcinomas (ISCC) without lymph node (LN) metastasis, and 20 ISCC with lymph node metastasis. We also used the reverse transcriptase-polymerase chain reaction technique to determine the expression of matriptase transcripts in normal cervical and ISCC tissues. The immunohistochemical staining showed that the expression of matriptase was undetectable in all normal cervical squamous epithelia, but had cytoplasmic and membranous staining in the normal endocervical glands. Staining gradually increased in accordance with the histopathologic grades from low-grade squamous intraepithelial lesions to high-grade squamous intraepithelial lesions and ISCC ( P < .001); matriptase was detected in most cases (95%) of ISCC. In addition, matriptase transcripts were expressed in all (n = 26) of the ISCC cases by microdissection and reverse transcriptase-polymerase chain reaction, whereas none of the normal squamous epithelia cases (n = 3) expressed matriptase transcripts. These results suggest that matriptase may play a significant role in the development of cervical carcinoma and may serve as a useful marker of the malignant transformation of cervical squamous cells. Further studies could potentially lead to the development of novel approaches for early detection and therapy for this disease.
胃蛋白酶是一种上皮来源的整合丝氨酸蛋白酶，与上皮肿瘤的进展有关。我们研究了胃蛋白酶的表达是否与宫颈肿瘤的进展相关。通过免疫组织化学，我们评估了89例福尔马林固定石蜡包埋宫颈组织中胃蛋白酶的表达，这些组织包括10例正常宫颈标本、19例低级别鳞状上皮内病变、20例高级别鳞状上皮内病变、20例无淋巴结转移的浸润性鳞状细胞癌（ISCC）以及20例有淋巴结转移的ISCC。我们还使用逆转录聚合酶链反应技术来确定正常宫颈组织和ISCC组织中胃蛋白酶转录本的表达。免疫组织化学染色显示，在所有正常宫颈鳞状上皮中均未检测到胃蛋白酶的表达，但在正常宫颈腺体内有细胞质和膜染色。染色随着组织病理学分级从低级别鳞状上皮内病变到高级别鳞状上皮内病变和ISCC逐渐增加（P <.001）；在大多数（95%）ISCC病例中检测到胃蛋白酶。此外，通过显微切割和逆转录聚合酶链反应，在所有（n = 26）ISCC病例中均表达胃蛋白酶转录本，而正常鳞状上皮病例（n = 3）均未表达胃蛋白酶转录本。这些结果表明，胃蛋白酶可能在宫颈癌的发生中起重要作用，并且可能作为宫颈鳞状细胞恶性转化的有用标志物。进一步的研究可能会导致开发出针对该疾病早期检测和治疗的新方法。
