Sakuma Nagahiko, Saeki Tomoaki, Yajima Kazuhiro, Hibino Takeshi, Yoshida Takayuki, Mizuno Hiromi, Mukai Seiji, Sakata Seiichiro, Kunimatsu Mitoshi, Kimura Genjiro
Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
J Nutr Sci Vitaminol (Tokyo). 2005 Apr;51(2):75-9. doi: 10.3177/jnsv.51.75.
The objective of the present study was to establish whether high-density lipoprotein 3 (HDL3) or high-density lipoprotein 2 (HDL2) might show an anti-oxidative effect on the acceleration of the oxidative modification of low-density lipoprotein (LDL) by ascorbic acid from measurement of the agarose gel electrophoretic mobility of LDL. LDL was incubated without adding transitional-metal ions for 48 or 96 h in phosphate-buffered saline (PBS) alone, with ascorbic acid (20 microg/mL), or with both ascorbic acid (20 microg/mL) and HDL3 (200 microg protein/mL). The LDL autoxidation occurred in PBS alone. Although ascorbic acid significantly suppressed oxidative modification of LDL after incubation for 48 h, the opposite was true after 96 h. However, since the anti-oxidative ability of HDL2 shows a weaker tendency than that of HDL3, both HDL3 and HDL2 significantly inhibited this acceleration of oxidative modification of LDL by ascorbic acid as assessed by electrophoretic mobility. If there is an augmented oxidative modification of LDL due to ascorbic acid in vivo, HDL3 or HDL2 may thus have an important role in inhibiting this ascorbic acid-accelerated oxidation of LDL.
本研究的目的是通过测量低密度脂蛋白（LDL）的琼脂糖凝胶电泳迁移率，确定高密度脂蛋白3（HDL3）或高密度脂蛋白2（HDL2）是否可能对维生素C加速低密度脂蛋白（LDL）的氧化修饰具有抗氧化作用。将LDL在不添加过渡金属离子的情况下，单独在磷酸盐缓冲盐水（PBS）中孵育48或96小时，或与维生素C（20微克/毫升）一起孵育，或与维生素C（20微克/毫升）和HDL3（200微克蛋白质/毫升）一起孵育。LDL的自动氧化仅在PBS中发生。虽然维生素C在孵育48小时后显著抑制了LDL的氧化修饰，但在96小时后情况相反。然而，由于HDL2的抗氧化能力比HDL3弱，通过电泳迁移率评估，HDL3和HDL2均显著抑制了维生素C对LDL氧化修饰的这种加速作用。如果体内由于维生素C导致LDL的氧化修饰增强，那么HDL3或HDL2可能在抑制维生素C加速的LDL氧化中起重要作用。
