Wang Yuebo, Xia Xinghua, Guo Yinlong
Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, 210093, Nanjing, People's Republic of China.
Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Science, 200032, Shanghai, People's Republic of China.
J Am Soc Mass Spectrom. 2005 Sep;16(9):1488-1492. doi: 10.1016/j.jasms.2005.04.014.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF MS) analysis of proteins in salt-containing solution was performed for the first time using porous anodic alumina (PAA) membrane as sample support. The resulting spectral quality of proteins under standard sample preparation conditions was superior to that of normal metal sample stages. Analysis of phosphate-doped protein solutions indicated that porous anodic alumina membranes as a target yielded better results than a metallic target for salt-containing solutions. Because of the biocompatibility of the PAA, proteins can be adsorbed on the PAA and thus a washing process can be introduced to remove the salts from the PAA target before MS analysis. This desalting step significantly enhanced spectral quality, and better signal-to-noise ratios were obtained. The present technique is promising for proteomics research.
首次使用多孔阳极氧化铝（PAA）膜作为样品支撑体，对含盐溶液中的蛋白质进行了基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）分析。在标准样品制备条件下，所得蛋白质的光谱质量优于普通金属样品台。对磷酸盐掺杂蛋白质溶液的分析表明，对于含盐溶液，作为靶标的多孔阳极氧化铝膜比金属靶标产生的结果更好。由于PAA的生物相容性，蛋白质可以吸附在PAA上，因此可以引入洗涤过程，在质谱分析之前从PAA靶标上去除盐分。这一脱盐步骤显著提高了光谱质量，并获得了更好的信噪比。本技术在蛋白质组学研究中具有广阔前景。
