Xu Yingda, Bruening Merlin L, Watson J Throck
Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.
Mass Spectrom Rev. 2003 Nov-Dec;22(6):429-40. doi: 10.1002/mas.10064.
High concentrations of contaminants such as salts and surfactants are often present in biological samples to solubilize or stabilize analytes such as proteins. Unfortunately, the presence of those contaminants often precludes direct analysis by MALDI-MS. Selective adsorption of analytes directly on modified MALDI probes, followed by rinsing to remove contaminants, overcomes this problem. This review focuses on various modifications of MALDI probes to allow the adsorption of proteins and DNA, even in a large excess of salt or surfactant. Interfaces deposited on the MALDI probes to adsorb analytes include films of commercial polymers, thin layers of matrix crystals, self-assembled monolayers, and ultrathin polymer films. Hydrophobic and ionic interactions both effect analyte adsorption on those interfaces, and patterned interfaces allow the concentration and purification of analyte molecules.
生物样品中常常存在高浓度的污染物，如盐和表面活性剂，用于溶解或稳定蛋白质等分析物。不幸的是，这些污染物的存在常常妨碍通过基质辅助激光解吸/电离质谱（MALDI-MS）进行直接分析。将分析物直接选择性吸附在改性的MALDI探针上，然后冲洗以去除污染物，可克服这一问题。本综述重点关注MALDI探针的各种改性，以实现蛋白质和DNA的吸附，即使在存在大量盐或表面活性剂的情况下。沉积在MALDI探针上用于吸附分析物的界面包括商业聚合物薄膜、基质晶体薄层、自组装单分子层和超薄聚合物薄膜。疏水相互作用和离子相互作用都会影响分析物在这些界面上的吸附，而图案化界面则可实现分析物分子的浓缩和纯化。
