Dashtiev Maxim, Azov Vladimir, Frankevich Vladimir, Scharfenberg Ludwig, Zenobi Renato
Department of Chemistry, ETH Honggerberg, Swiss Federal Institute of Technology, HCI E 329, CH-8093, Zurich, Switzerland.
J Am Soc Mass Spectrom. 2005 Sep;16(9):1481-1487. doi: 10.1016/j.jasms.2005.04.012.
Fluorescence resonance energy transfer (FRET) is a distance-sensitive method that correlates changes in fluorescence intensity with conformational changes, for example, of biomolecules in the cellular environment. Applied to the gas phase in combination with Fourier transform ion cyclotron resonance mass spectrometry, it opens up possibilities to define structural/conformational properties of molecular ions, in the absence of solvent, and without the need for purification of the sample. For successfully observing FRET in the gas phase it is important to find suitable fluorophores. In this study several fluorescent dyes were examined, and the correlation between solution-phase and gas-phase fluorescence data were studied. For the first time, FRET in the gas phase is demonstrated unambiguously.
荧光共振能量转移（FRET）是一种对距离敏感的方法，它将荧光强度的变化与构象变化相关联，例如细胞环境中生物分子的构象变化。与傅里叶变换离子回旋共振质谱联用应用于气相时，它为在无溶剂且无需样品纯化的情况下定义分子离子的结构/构象性质开辟了可能性。为了在气相中成功观察到FRET，找到合适的荧光团很重要。在本研究中，对几种荧光染料进行了检测，并研究了溶液相和气相荧光数据之间的相关性。首次明确证明了气相中的FRET。
