Fritzsch B, Muirhead K A, Feng Feng, Gray B D, Ohlsson-Wilhelm B M
Department of Biomedical Sciences, Creighton University, Omaha, NE 68178, USA.
Brain Res Bull. 2005 Aug 15;66(3):249-58. doi: 10.1016/j.brainresbull.2005.05.016.
We describe here diffusion and imaging properties of three new lipophilic tracers, NeuroVue Maroon (near infrared), NeuroVue Red and NeuroVue Green. Using pair-wise comparisons between the new dyes and existing dyes (DiI, DiA, DiD, DiO, PKH2, PKH26) applied to the left and the right side of fixed spinal cord preparations, we show that NeuroVue Maroon (excitation maximum 647 nm) surpasses all other dyes in this study in signal to noise ratio. We also present data showing the utility of these new dyes for both double labeling and triple labeling in combination with each other or existing lipophilic tracers. Using mice bearing the PLP-eGFP transgene, we demonstrate that either NeuroVue Maroon or NeuroVue Red can readily be combined with eGFP labeling. Double labeling experiments using NeuroVue Red and eGFP allowed us to demonstrate that every fiber in the neonatal ear is surrounded by developing Schwann cells.
我们在此描述三种新型亲脂性示踪剂NeuroVue Maroon（近红外）、NeuroVue Red和NeuroVue Green的扩散及成像特性。通过对固定脊髓制剂左右两侧应用的新型染料与现有染料（DiI、DiA、DiD、DiO、PKH2、PKH26）进行两两比较，我们发现NeuroVue Maroon（激发最大值647nm）在本研究中的信噪比超过所有其他染料。我们还展示了数据，表明这些新型染料在相互组合或与现有亲脂性示踪剂组合用于双重标记和三重标记方面的效用。利用携带PLP - eGFP转基因的小鼠，我们证明NeuroVue Maroon或NeuroVue Red都能很容易地与eGFP标记相结合。使用NeuroVue Red和eGFP进行的双重标记实验使我们能够证明新生小鼠耳部的每根纤维都被正在发育的施万细胞所包围。
