Vaiopoulou E, Melidis P, Kampragou E, Aivasidis A
Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100 Xanthi, Greece.
Biosens Bioelectron. 2005 Aug 15;21(2):365-71. doi: 10.1016/j.bios.2004.10.022. Epub 2004 Dec 15.
This paper demonstrates the functionality, laboratory testing and field application of a microbial sensor that is capable of monitoring the organic pollution extent of wastewaters both off-line in a laboratory and on-line in a wastewater treatment plant. The biosensor was first developed in the laboratory using synthetic wastewater and then applied to monitor the effluent of the unit. The basic working principle of the biosensor is based on the on-line measurement of CO2 concentration in the off gas produced during carbon compound degradation by microbial respiration activities. CO2 concentration under operation conditions (constant oxygen flow rate, residence time and pH) is closely related to the extent of organic pollution (biochemical oxygen demand, chemical oxygen demand). CO2 monitoring is carried out by an infrared spectrometer, whereas current organic pollution is determined off-line according to the conventional 5-day lasting BOD analysis. Off gas analysis of CO2 concentration strongly correlates with off-line biochemical oxygen demand measurements allowing continuous on-line monitoring of the organic load within a wastewater treatment plant. Thus, real time process control and operation become feasible.
本文展示了一种微生物传感器的功能、实验室测试及现场应用，该传感器能够在实验室离线和污水处理厂在线监测废水的有机污染程度。这种生物传感器首先在实验室使用合成废水进行开发，然后应用于监测该单元的出水。生物传感器的基本工作原理基于对微生物呼吸活动降解碳化合物过程中产生的废气中二氧化碳浓度的在线测量。运行条件下（恒定氧气流速、停留时间和pH值）的二氧化碳浓度与有机污染程度（生化需氧量、化学需氧量）密切相关。二氧化碳监测通过红外光谱仪进行，而当前的有机污染则根据传统的五日生化需氧量分析离线测定。对废气中二氧化碳浓度的分析与离线生化需氧量测量结果高度相关，从而能够对污水处理厂内的有机负荷进行连续在线监测。因此，实时过程控制和运行变得可行。
