Jedrzejczak P, Pawelczyk L, Taszarek-Hauke G, Kotwicka M, Warchoł W, Kurpisz M
Infertility and Reproductive Endocrinology, University of Medical Sciences, Poznan, Poland.
Andrologia. 2005 Jun;37(2-3):72-82. doi: 10.1111/j.1439-0272.2005.00657.x.
A proportion of fertilized oocytes during classical in vitro fertilization (IVF) procedure was analysed depending on the following factors: number of mature oocytes, seminological criteria such as sperm morphology in raw semen and after its selection in a density gradient (six structural defects of a male gamete were taken into consideration), sperm concentration, motility parameters according to World Health Organization criteria and the functional tests: hypo-osmotic swelling assay and acrosomal reaction induced by calcium ionophore. Evaluation of DNA content in sperm by image cytometry and determination of malonyldialdehydes in seminal plasma were also performed. Seventy-nine semen samples from patients undergoing IVF were assessed. Apart from significant correlations obtained for selected semen parameters and proportion of fertilized eggs, logistic regression analysis showed that the best predictive factors for oocyte fertilization were normal morphology of sperm before and after gradient selection, grade B and C of sperm movement in raw semen, and DNA content after density gradient centrifugation, which all accounted for 76.7% of fertilization predictive value.
在经典体外受精（IVF）过程中，根据以下因素对一部分受精卵进行了分析：成熟卵母细胞的数量、精液学标准，如原精液中的精子形态以及在密度梯度中筛选后的精子形态（考虑了雄配子的六种结构缺陷）、精子浓度、根据世界卫生组织标准的活力参数以及功能测试：低渗肿胀试验和钙离子载体诱导的顶体反应。还通过图像细胞术评估了精子中的DNA含量，并测定了精浆中的丙二醛。对79例接受IVF患者的精液样本进行了评估。除了所选精液参数与受精卵比例之间存在显著相关性外，逻辑回归分析表明，卵母细胞受精的最佳预测因素是梯度选择前后精子的正常形态、原精液中B级和C级精子运动以及密度梯度离心后的DNA含量，这些因素共同占受精预测值的76.7%。
