Joshi N, Kodwany G, Balaiah D, Parikh M, Parikh F
Jaslok Hospital and Research Center, Bombay, India.
Int J Fertil Menopausal Stud. 1996 Jan-Feb;41(1):46-52.
To study the relationship of sperm motion characteristics, sperm morphology, and hypo-osmotic swelling test with fertilization rates in vitro.
Computer-assisted measures of fresh seminal and processed sperm preparations, sperm morphology, and hypo-osmotic swelling test results were assessed for predicting fertilization by step-wise regression analysis.
In vitro fertilization laboratory, department of infertility management, university affiliated-hospital.
None.
One hundred and two couples who underwent IVF were studied.
Computer-assisted sperm motion variables in semen and following semen processing in capacitating media; hypo-osmotic swelling of sperm tails before and after semen processing; morphology of sperm before and after processing; fertilization of oocytes as evidenced by presence of two pronuclei as the end point.
Various sperm motion parameters, hypo-osmotic swelling test results, and normal morphology of sperm were directly correlated to fertilization as judged by the Karl-Pearsons test. However, in step-wise regression analysis, normal morphology of sperm from seminal fraction exhibited 61% correlation with fertilization rates (P < .001). In step 2, normal morphology along with percent motility exhibited a 64% correlation with fertilization rates (P < .001). In step 3, normal morphology, percent motility, and linearity showed a 67% correlation with fertilization rates (P < .001). The hypo-osmotic swelling test did not predict fertilization rates (regression coefficient = 0.066, P = 0.474). In the processed sample preparations, normal sperm morphology showed a 72% correlation with fertilization rate (P < .001). In step 2, normal morphology along with curvilinear velocity exhibited a 77% correlation with fertilization rates (P < .001). In step 3, normal morphology, curvilinear velocity, and average path velocity showed a 79% correlation with fertilization rates (P < .001). The hypo-osmotic swelling test did not predict fertilization rates (regression coefficient = 0.076, P = 0.512).
Morphology of sperm and computer-assisted sperm motion variables, such as motility, linearity, curvilinear velocity, and average path velocity, may serve as prognostic indicators for fertilization potential of sperm. The hypo-osmotic swelling test may describe only physiological intactness, rather than the fertilization potential of sperm. The results suggest that objective analysis of sperm motion characteristics and precise sperm morphology may form a first, and obligatory, step for critical evaluation of patients before they start IVF treatment.
研究精子运动特征、精子形态及低渗肿胀试验与体外受精率的关系。
通过逐步回归分析评估新鲜精液及处理后的精子制剂的计算机辅助测量结果、精子形态及低渗肿胀试验结果，以预测受精情况。
大学附属医院不孕不育治疗科的体外受精实验室。
无。
对102例行体外受精的夫妇进行研究。
精液及在获能培养基中处理后的精液中计算机辅助精子运动变量；精液处理前后精子尾部的低渗肿胀情况；处理前后精子的形态；以出现两个原核作为终点来证明卵母细胞的受精情况。
通过卡尔 - 皮尔逊检验判断，各种精子运动参数、低渗肿胀试验结果及精子正常形态与受精直接相关。然而，在逐步回归分析中，精液中精子的正常形态与受精率的相关性为61%（P <.001）。在第二步中，正常形态与活动率百分比与受精率的相关性为64%（P <.001）。在第三步中，正常形态、活动率百分比及直线性与受精率的相关性为67%（P <.001）。低渗肿胀试验不能预测受精率（回归系数 = 0.066，P = 0.474）。在处理后的样本制剂中，正常精子形态与受精率的相关性为72%（P <.001）。在第二步中，正常形态与曲线速度与受精率的相关性为77%（P <.001）。在第三步中，正常形态、曲线速度及平均路径速度与受精率的相关性为79%（P <.001）。低渗肿胀试验不能预测受精率（回归系数 = 0.076，P = 0.512）。
精子形态及计算机辅助精子运动变量，如活动率、直线性、曲线速度及平均路径速度，可作为精子受精潜力的预后指标。低渗肿胀试验可能仅描述精子的生理完整性，而非其受精潜力。结果表明，对精子运动特征进行客观分析及精确评估精子形态可能是患者开始体外受精治疗前进行关键评估的首要且必要步骤。
