Hasanudin Udin, Faisal Muhammad, Daimon Hiroyuki, Fujie Koichi
Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi, 441-8580, Japan.
Appl Microbiol Biotechnol. 2006 Jan;69(5):506-9. doi: 10.1007/s00253-005-0008-8. Epub 2005 Jul 19.
Supercritical carbon dioxide (scCO2) was applied to extract microbial quinones from activated sludge. Identification and analysis was then performed using high-performance liquid chromatography (HPLC) equipped with ultraviolet-visible (UV-Vis) detector and photodiode array detector (PDA). Extracted microbial quinones were trapped and separated as menaquinones (MK) and ubiquinones (Q) species using two Sep-Pak Plus Silica cartridges joined in series. Four ubiquinones and 12 menaquinones species were identified in 0.1 g dried activated sludge based on retention time and spectrum analysis. Among the tested various polar solvents, methanol showed to be the best modifier, based on the highest total quinone content extracted and the lowest dissimilarity index. The diversity index of quinone and the number of quinone species using methanol-modified scCO2 were similar to that of the conventional method (organic solvent extraction).
采用超临界二氧化碳（scCO2）从活性污泥中提取微生物醌。然后使用配备紫外可见（UV-Vis）检测器和光电二极管阵列检测器（PDA）的高效液相色谱（HPLC）进行鉴定和分析。使用两个串联的Sep-Pak Plus硅胶柱将提取的微生物醌捕获并分离为甲基萘醌（MK）和泛醌（Q）种类。基于保留时间和光谱分析，在0.1 g干燥活性污泥中鉴定出4种泛醌和12种甲基萘醌。在所测试的各种极性溶剂中，基于提取的总醌含量最高和差异指数最低，甲醇被证明是最佳改性剂。使用甲醇改性scCO2的醌多样性指数和醌种类数量与传统方法（有机溶剂萃取）相似。
